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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

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1437-4315
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Volume 387, Issue 5

Issues

Transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth Galleria mellonella, confers resistance to pathogenic fungi in tobacco

Gregor Langen
  • Institute of Phytopathology and Applied Zoology, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jafargholi Imani
  • Institute of Phytopathology and Applied Zoology, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Boran Altincicek
  • Institute of Phytopathology and Applied Zoology, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gernot Kieseritzky
  • Department of Biology, Chemistry and Pharmacy, Institute of Chemistry and Biochemistry, Free University of Berlin, Takustr. 6, D-14195 Berlin, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Karl-Heinz Kogel
  • Institute of Phytopathology and Applied Zoology, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Andreas Vilcinskas
  • Institute of Phytopathology and Applied Zoology, Justus-Liebig-University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2006-06-01 | DOI: https://doi.org/10.1515/BC.2006.071

Abstract

A cDNA encoding gallerimycin, a novel antifungal peptide from the greater wax moth Galleria mellonella, was isolated from a cDNA library of genes expressed during innate immune response in the caterpillars. Upon ectopic expression of gallerimycin in tobacco, using Agrobacterium tumefaciens as a vector, gallerimycin conferred resistance to the fungal pathogens Erysiphe cichoracearum and Sclerotinia minor. Quantification of gallerimycin mRNA in transgenic tobacco by real-time PCR confirmed transgenic expression under control of the inducible mannopine synthase promoter. Leaf sap and intercellular washing fluid from transgenic tobacco inhibited in vitro germination and growth of the fungal pathogens, demonstrating that gallerimycin is secreted into intercellular spaces. The feasibility of the use of gallerimycin to counteract fungal diseases in crop plants is discussed.

Keywords: Erysiphe cichoracearum; fungal resistance; Galleria mellonella; gallerimycin; mas promoter; Sclerotinia minor; tobacco

About the article

Corresponding author


Received: January 13, 2006

Accepted: March 7, 2006

Published Online: 2006-06-01

Published in Print: 2006-05-01


Citation Information: Biological Chemistry, Volume 387, Issue 5, Pages 549–557, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2006.071.

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