Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

249,00 € / $374.00 / £187.00*

See all formats and pricing



Select Volume and Issue


30,00 € / $42.00 / £23.00

Get Access to Full Text

Human kallikrein 10, a predictive marker for breast cancer

Ying Zhang1 / Ishfaq Bhat2 / Musheng Zeng3 / Goyal Jayal4 / David E. Wazer5 / Hamid Band6 / Vimla Band7








Corresponding author

Citation Information: Biological Chemistry. Volume 387, Issue 6, Pages 715–721, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: 10.1515/BC.2006.090, June 2006

Publication History

Published Online:


Our laboratory is involved in identifying genes that can be used as early diagnostic or prognostic markers in breast cancer. We previously identified a gene (NES1) that is expressed in normal but not in transformed mammary epithelial cells (MECs). NES1 is located on chromosome 19q13.4 within the kallikrein locus and thus was designated as human kallikrein 10 (hK10), although we have been unable to detect any protease activity. Importantly, hK10 expression is decreased in a majority of breast cancer cell lines. Transfection of hK10 into hK10-negative breast cancer cells reduces the tumorigenicity. Using methylation-specific PCR and subsequent sequencing, we demonstrate a strong correlation between hypermethylation of hK10 and loss of mRNA expression. Further analysis showed that essentially 100% of normal breast specimens had hK10 expression, whereas 46% of ductal carcinoma in situ (DCIS) and the majority of infiltrating ductal carcinoma (IDC) samples lacked the hK10 mRNA. Importantly, hK10-negative DCIS diagnosed at the time of biopsy were subsequently diagnosed as IDC at the time of definitive surgery. It has been shown that hK10 protein expression is regulated by steroids. In addition to breast cancers, hK10 is downregulated in cervical cancer, prostate cancer and acute lymphocytic leukemia, whereas it is upregulated in ovarian cancers. These results point to the paradoxical role of hK10 in human cancers and underscore the importance of further studies of this kallikrein.

Keywords: breast cancer; hK10; hypermethylation; kallikreins; predictive markers; serine protease

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Weiwei Li, Yi Zhao, Lina Ren, and Xin Wu
Medical Oncology, 2014, Volume 31, Number 10
Attila T Lorincz
Expert Review of Molecular Diagnostics, 2014, Volume 14, Number 3, Page 293
Lindsay Ewan King, Xiaogang Li, Kane Cheikh Saad Bouh, Marc Pedneault, and Chee-Wui Chu
Clinical Biochemistry, 2007, Volume 40, Number 13-14, Page 1057
Wei Huang, Jie Zhong, Lin-Yun Wu, Li-Fen Yu, Xiang-long Tian, Yi-Fan Zhang, and Biao Li
Cancer Letters, 2007, Volume 251, Number 1, Page 78
Katerina Oikonomopoulou, Ihor Batruch, Chris R. Smith, Antoninus Soosaipillai, Eleftherios P. Diamandis, and Morley D. Hollenberg
Biological Chemistry, 2010, Volume 391, Number 4
Manish Mani Subramaniam, Jason Yongsheng Chan, Richie Soong, Kosei Ito, Yoshiaki Ito, Khay Guan Yeoh, Manuel Salto-Tellez, and Thomas Choudary Putti
Breast Cancer Research and Treatment, 2009, Volume 113, Number 1, Page 113
F Rückert, M Hennig, C D Petraki, D Wehrum, M Distler, A Denz, M Schröder, G Dawelbait, H Kalthoff, H-D Saeger, E P Diamandis, C Pilarsky, and R Grützmann
British Journal of Cancer, 2008, Volume 99, Number 9, Page 1484
Wun-Shaing W Chang, Ruey-Hwang Chou, Cheng-Wen Wu, and Jang-Yang Chang
Expert Opinion on Therapeutic Patents, 2007, Volume 17, Number 10, Page 1227
Jing Yu, Feng Liu, Shu-Jian Cui, Yan Liu, Zheng-Yu Song, Hui Cao, Feng-E Chen, Wei-Jun Wang, Tao Sun, and Fang Wang
PROTEOMICS, 2008, Volume 8, Number 17, Page 3667

Comments (0)

Please log in or register to comment.