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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

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IMPACT FACTOR 2016: 3.273

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1437-4315
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Volume 387, Issue 9

Issues

Sequence determination of lychnin, a type 1 ribosome-inactivating protein from Lychnis chalcedonica seeds

Angela Chambery
  • Dipartimento di Scienze della Vita, Seconda Università di Napoli, Via Vivaldi 43, I-81100 Caserta, Italy
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/ Anna de Donato
  • Dipartimento di Scienze della Vita, Seconda Università di Napoli, Via Vivaldi 43, I-81100 Caserta, Italy
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/ Andrea Bolognesi
  • Dipartimento di Patologia Sperimentale, Università di Bologna, Via S. Giacomo 14, I-40126 Bologna, Italy
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/ Letizia Polito
  • Dipartimento di Patologia Sperimentale, Università di Bologna, Via S. Giacomo 14, I-40126 Bologna, Italy
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/ Fiorenzo Stirpe
  • Dipartimento di Patologia Sperimentale, Università di Bologna, Via S. Giacomo 14, I-40126 Bologna, Italy
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/ Augusto Parente
  • Dipartimento di Scienze della Vita, Seconda Università di Napoli, Via Vivaldi 43, I-81100 Caserta, Italy
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Published Online: 2006-09-14 | DOI: https://doi.org/10.1515/BC.2006.156

Abstract

The complete amino acid sequence of lychnin, a type 1 ribosome-inactivating protein (RIP) isolated from Lychnis chalcedonica seeds, has been determined by automated Edman degradation and ESI-QTOF mass spectrometry. Lychnin consists of 234 amino acid residues with a molecular mass of 26 131.14 Da. All amino acid residues involved in the formation of the RIP active site (Tyr69, Tyr119, Glu170, Arg173 and Trp203) are fully conserved. Furthermore, a fast MALDI-TOF experiment showed that two out of three cysteinyl residues (Cys32 and Cys115) form a disulfide bridge, while Cys214 is in the thiol form, which makes it suitable for linking carrier molecules to generate immunotoxins and other conjugates.

Keywords: Edman degradation; electrospray dual-ESI-sprayer system; immunotoxin; ribosome-inactivating protein

About the article

Corresponding author


Received: March 7, 2006

Accepted: May 30, 2006

Published Online: 2006-09-14

Published in Print: 2006-09-01


Citation Information: Biological Chemistry, Volume 387, Issue 9, Pages 1261–1266, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2006.156.

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