Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2015: 2.710
Rank 142 out of 289 in category Biochemistry & Molecular Biology in the 2015 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

See all formats and pricing



Select Volume and Issue


Characterization of rat BLOS2/Ceap, a putative yeast She3 homolog, as interaction partner of apoptosis antagonizing transcription factor/Che-1

Andrea Felten
  • 1Institute of Genetics, University of Bonn, Roemerstr. 164, D-53117 Bonn, Germany
    The first two authors contributed equally to this work.
/ Peter Leister
  • 2Institute of Genetics, University of Bonn, Roemerstr. 164, D-53117 Bonn, Germany
/ Sven Burgdorf
  • 3Institute of Genetics, University of Bonn, Roemerstr. 164, D-53117 Bonn, Germany and Present address: Institute of Molecular Medicine and Experimental Immunology, University of Bonn, Sigmund-Freud-Str. 25, D-53105 Bonn, Germany.
/ Lutz Uhlmann
  • 4Institute of Genetics, University of Bonn, Roemerstr. 164, D-53117 Bonn, Germany
/ Karl Heinz Scheidtmann
  • 5Institute of Genetics, University of Bonn, Roemerstr. 164, D-53117 Bonn, Germany
Published Online: 2007-06-01 | DOI: https://doi.org/10.1515/BC.2007.073


AATF/Che-1 is a coactivator of several transcription factors, including steroid hormone receptors. In search of novel interaction partners of AATF, we identified BLOS2 (BLOC1S2, also termed Ceap) from a rat cDNA library. BLOS2 is extremely conserved with a high degree of homology to yeast She3p. The clone isolated represents a splice variant encoding a polypeptide of 168 residues. Rat BLOS2 mRNA is highly expressed in brain and testis and at lower levels in other tissues, but not in skeletal or smooth muscle. Expression as a tagged fusion protein revealed predominant cytoplasmic, but also nuclear localization. In the cytoplasm, BLOS2 fusion proteins exhibit diffuse, filamentous, or dotted distribution, with the latter partially co-localizing with recycling endosomes. In addition, BLOS2 localizes to centrosomes or the pericentrosomal region. Moreover, BLOS2 co-localizes with myosin V globular tail domains in vesicle-like structures. However, a direct interaction could not be demonstrated. In transactivation assays, BLOS2 enhanced transcription from androgen receptor and p53-responsive promoters. However, this enhancement correlated with accumulation of both androgen receptor and p53, suggesting that BLOS2 has a stabilizing effect on these transcription factors. We propose that BLOS2 functions as an adapter in processes such as protein and vesicle processing and transport, and perhaps transcription.

Keywords: AATF/Che-1; androgen receptor; BLOC1S2/Ceap/RSEP; myosin V; p53; She3p

Corresponding author

Received: 2006-10-24

Accepted: 2007-03-19

Published Online: 2007-06-01

Citation Information: Biological Chemistry. Volume 388, Issue 6, Pages 569–582, ISSN (Online) 14316730, ISSN (Print) 14374315, DOI: https://doi.org/10.1515/BC.2007.073, June 2007

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Xin-Jie Zhuang, Yu-Qiang Shi, Bo Xu, Lei Chen, Wen-Hao Tang, Jin Huang, Ying Lian, Ping Liu, and Jie Qiao
Cell Cycle, 2014, Volume 13, Number 14, Page 2230
Verónica T. Cheli and Esteban C. Dell’Angelica
Traffic, 2010, Volume 11, Number 5, Page 579
I. A. Rodriguez-Fernandez and E. C. Dell’Angelica
Journal of Inherited Metabolic Disease, 2009, Volume 32, Number 2, Page 190
P Leister, A Felten, A I Chasan, and K H Scheidtmann
Oncogene, 2008, Volume 27, Number 23, Page 3292
Georg Gdynia, Judith Lehmann-Koch, Sebastian Sieber, Katrin E. Tagscherer, Anne Fassl, Hanswalter Zentgraf, Shu-Ichi Matsuzawa, John C. Reed, and Wilfried Roth
Apoptosis, 2008, Volume 13, Number 3, Page 437

Comments (0)

Please log in or register to comment.