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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2016: 1.679
Source Normalized Impact per Paper (SNIP) 2016: 0.800

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ISSN
1437-4315
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Volume 388, Issue 6 (Jun 2007)

Issues

Competitive displacement of full-length HIV-1 Nef from the Hck SH3 domain by a high-affinity artificial peptide

Thomas Stangler
  • 1Institut für Physikalische Biologie and BMFZ, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany and Institut für Neurowissenschaften und Biophysik INB-2, Forschungszentrum Jülich, D-52425 Jülich, Germany
    The first two authors contributed equally to this work.
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/ Tuyen Tran
  • 2Institut für Physikalische Biologie and BMFZ, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany, Institut für Neurowissenschaften und Biophysik INB-2, Forschungszentrum Jülich, D-52425 Jülich, Germany and Present address: Institut für Biochemie, Westfälische Wilhelms-Universität Münster, Wilhelm-Klemm-Str. 2, D-48149 Münster, Germany.
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/ Silke Hoffmann
  • 3Institut für Physikalische Biologie and BMFZ, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany and Institut für Neurowissenschaften und Biophysik INB-2, Forschungszentrum Jülich, D-52425 Jülich, Germany
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/ Holger Schmidt
  • 4Institut für Physikalische Biologie and BMFZ, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany and Institut für Neurowissenschaften und Biophysik INB-2, Forschungszentrum Jülich, D-52425 Jülich, Germany
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/ Esther Jonas
  • 5Institut für Physikalische Biologie and BMFZ, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany and Institut für Neurowissenschaften und Biophysik INB-2, Forschungszentrum Jülich, D-52425 Jülich, Germany
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/ Dieter Willbold
  • 6Institut für Physikalische Biologie and BMFZ, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany and Institut für Neurowissenschaften und Biophysik INB-2, Forschungszentrum Jülich, D-52425 Jülich, Germany
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Published Online: 2007-06-01 | DOI: https://doi.org/10.1515/BC.2007.075

Abstract

We studied the interaction of the artificial 12-aa proline-rich peptide PD1 with the SH3 domain of the hematopoietic cell kinase Hck and the peptide's potency in competitively displacing HIV-1 Nef from the Hck SH3 domain. PD1 was obtained from a phage display screen and exhibits exceptional affinity for the Hck SH3 domain (K d=0.23 μM). Competition experiments using NMR spectroscopy demonstrate that the peptide even displaces Nef from Hck SH3 and allow for estimation of the Nef-Hck SH3 dissociation constant (K d=0.44 μM), the strongest SH3 ligand interaction known so far. Consequences of this study for novel antiviral concepts are discussed.

Keywords: competition; Hck; HIV; Nef; NMR; protein-peptide interaction; SH3

About the article

Corresponding author


Received: 2007-01-26

Accepted: 2007-03-20

Published Online: 2007-06-01


Citation Information: Biological Chemistry, ISSN (Online) 14316730, ISSN (Print) 14374315, DOI: https://doi.org/10.1515/BC.2007.075.

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