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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

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Volume 389, Issue 12


Quantitative expression analysis and prognostic significance of the novel apoptosis-related gene BCL2L12 in colon cancer

Christos K. Kontos
  • 1Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, GR-15701 Athens, Greece
  • Other articles by this author:
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/ Iordanis N. Papadopoulos
  • 24th Department of Surgery, ‘Attikon’ University Hospital, Faculty of Medicine, University of Athens, GR-15701 Athens, Greece
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/ Andreas Scorilas
  • 3Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, GR-15701 Athens, Greece
  • Other articles by this author:
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Published Online: 2008-10-09 | DOI: https://doi.org/10.1515/BC.2008.173


Apoptosis is a tightly regulated process that plays a critical role in many biological events. Members of the BCL2 (Bcl-2) family of apoptosis-related genes have been found to be differentially expressed in various malignancies and have been proposed as prognostic tumor biomarkers. We have recently discovered and cloned a new member of the BCL2 gene family, BCL2L12, expressed in colon tissues. Here we have investigated expression of the BCL2L12 gene in colon cancer tissues and assessed its prognostic value. Total RNA was isolated from 96 specimens of malignant colon tissue. After testing the RNA quality, cDNA was prepared by reverse transcription. A highly sensitive real-time PCR method for BCL2L12 mRNA quantification was developed using SYBR® Green chemistry. GAPDH served as a housekeeping gene. Relative quantification analysis was performed using the comparative CT method (2-ΔΔCT). High BCL2L12 expression levels were found in smaller (≤5 cm, p=0.027) and well-differentiated tumors (p=0.034), as well as in early-stage tumors (p=0.039). Survival analysis demonstrated that patients with BCL2L12-positive colon tumors have significantly longer disease-free survival and overall survival (p=0.015 and p=0.027, respectively). Our results suggest that BCL2L12 gene expression may represent a potential new biomarker for colon cancer.

Keywords: apoptosis; BCL2 (Bcl-2) family; BCL2L12; real-time PCR; tumor biomarkers

About the article

Corresponding author

Received: 2008-06-17

Accepted: 2008-09-08

Published Online: 2008-10-09

Published in Print: 2008-12-01

Citation Information: Biological Chemistry, Volume 389, Issue 12, Pages 1467–1475, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2008.173.

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