Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2015: 2.710
Rank 142 out of 289 in category Biochemistry & Molecular Biology in the 2015 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

See all formats and pricing



Select Volume and Issue


Regulation of the expression of components of the exocytotic machinery of insulin-secreting cells by microRNAs

Pascal Lovis
  • 1Department of Cell Biology and Morphology, Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland
/ Sonia Gattesco
  • 2Department of Cell Biology and Morphology, Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland
/ Romano Regazzi
  • 3Department of Cell Biology and Morphology, Faculty of Biology and Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland
Published Online: 2008-03-03 | DOI: https://doi.org/10.1515/BC.2008.026


Fine-tuning of insulin secretion from pancreatic β-cells participates in blood glucose homeostasis. Defects in this process can lead to chronic hyperglycemia and diabetes mellitus. Several proteins controlling insulin exocytosis have been identified, but the mechanisms regulating their expression remain poorly understood. Here, we show that two non-coding microRNAs, miR124a and miR96, modulate the expression of proteins involved in insulin exocytosis and affect secretion of the β-cell line MIN6B1. miR124a increases the levels of SNAP25, Rab3A and synapsin-1A and decreases those of Rab27A and Noc2. Inhibition of Rab27A expression is mediated by direct binding to the 3′-untranslated region of Rab27A mRNA. The effect on the other genes is indirect and linked to changes in mRNA levels. Over-expression of miR124a leads to exaggerated hormone release under basal conditions and a reduction in glucose-induced secretion. miR96 increases mRNA and protein levels of granuphilin, a negative modulator of insulin exocytosis, and decreases the expression of Noc2, resulting in lower capacity of MIN6B1 cells to respond to secretagogues. Our data identify miR124a and miR96 as novel regulators of the expression of proteins playing a critical role in insulin exocytosis and in the release of other hormones and neurotransmitters.

Keywords: exocytosis; insulin secretion; microRNA; neurotransmitter release; pancreatic β-cell

Corresponding author

Received: 2007-08-03

Accepted: 2007-11-27

Published Online: 2008-03-03

Published in Print: 2008-03-01

Citation Information: Biological Chemistry. Volume 389, Issue 3, Pages 305–312, ISSN (Online) 14374315, ISSN (Print) 14316730, DOI: https://doi.org/10.1515/BC.2008.026, March 2008

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Ya‑Nan Sun, Si Li, Yun‑Tao Zhou, Jia Liu, Luo‑Bing Tian, Yan‑Feng Zhen, and Hui Fang
Molecular Medicine Reports, 2015
Parnika Bhatia, Shikha Raina, Jeetender Chugh, and Shilpy Sharma
Biomarkers in Medicine, 2015
Ying Zheng, Zhen Wang, Yiting Tu, Hongwei Shen, Zhijie Dai, Jian Lin, and Zhiguang Zhou
Laboratory Investigation, 2015
Ihn Suk Lee, Ki Cheol Park, Keum-Jin Yang, Hyunsu Choi, Yi Sun Jang, Jong Min Lee, and Hye Soo Kim
Obesity Research & Clinical Practice, 2015
Munish Kumar, Sayantan Nath, Himanshu K. Prasad, G. D. Sharma, and Yong Li
Protein & Cell, 2012, Volume 3, Number 10, Page 726
Ruth A. Singer, Luis Arnes, and Lori Sussel
Current Opinion in Endocrinology & Diabetes and Obesity, 2015, Volume 22, Number 2, Page 77
E. Roggli, S. Gattesco, D. Caille, C. Briet, C. Boitard, P. Meda, and R. Regazzi
Diabetes, 2012, Volume 61, Number 7, Page 1742
Lena Eliasson, Fernando Abdulkader, Matthias Braun, Juris Galvanovskis, Michael B. Hoppa, and Patrik Rorsman
The Journal of Physiology, 2008, Volume 586, Number 14, Page 3313
Honghao Zhang, Yujie Guo, Amarjit Mishra, Deming Gou, Narendranath Reddy Chintagari, and Lin Liu
FEBS Letters, 2015, Volume 589, Number 1, Page 172
Guido Sebastiani, Agnese Po, Evelina Miele, Giuliana Ventriglia, Elena Ceccarelli, Marco Bugliani, Lorella Marselli, Piero Marchetti, Alberto Gulino, Elisabetta Ferretti, and Francesco Dotta
Acta Diabetologica, 2015, Volume 52, Number 3, Page 523
Jinghui Zhou, Yuhuan Meng, Shuai Tian, Junhui Chen, Mingyu Liu, Min Zhuo, Yu Zhang, Hongli Du, and Xiaoning Wang
Journal of Diabetes Research, 2014, Volume 2014, Page 1
Paul S Kelly, Colin Clarke, Martin Clynes, and Niall Barron
Pharmaceutical Bioprocessing, 2014, Volume 2, Number 4, Page 323
Xing Xu, Gaihong Wang, Tingting Zhou, Lili Chen, Jing Chen, and Xu Shen
Expert Opinion on Drug Discovery, 2014, Volume 9, Number 9, Page 1047
Nathan L. Price, Cristina M. Ramírez, and Carlos Fernández-Hernando
Critical Reviews in Clinical Laboratory Sciences, 2014, Volume 51, Number 6, Page 305
Chiranjib Chakraborty, C. George Priya Doss, Sanghamitra Bandyopadhyay, and Govindasamy Agoramoorthy
Wiley Interdisciplinary Reviews: RNA, 2014, Page n/a
H. Chen, H.-Y. Lan, D. H. Roukos, and W. C. Cho
Journal of Endocrinology, 2014, Volume 222, Number 1, Page R1
L. Eliasson and J. L. S. Esguerra
Acta Physiologica, 2014, Volume 211, Number 2, Page 273
An Hendrix and Olivier De Wever
International Journal of Molecular Sciences, 2013, Volume 14, Number 5, Page 9883
Valérie Plaisance, Gérard Waeber, Romano Regazzi, and Amar Abderrahmani
Journal of Diabetes Research, 2014, Volume 2014, Page 1
Amedeo Vetere, Amit Choudhary, Sean M. Burns, and Bridget K. Wagner
Nature Reviews Drug Discovery, 2014, Volume 13, Number 4, Page 278
Raihana Maqbool and Mahboob Ul Hussain
Cell and Tissue Research, 2014, Volume 358, Number 1, Page 1
Xueling Li
Gene, 2014, Volume 533, Number 1, Page 1
Kasey C. Vickers, Kerry‑Anne Rye, and Fatiha Tabet
Clinical Science, 2014, Volume 126, Number 3, Page 183
Elizabeth R. Gilbert and Dongmin Liu
Epigenetics, 2012, Volume 7, Number 8, Page 841
Charlotte Hinault, Olivier Dumortier, and Emmanuel Van Obberghen
médecine/sciences, 2013, Volume 29, Number 8-9, Page 785
Olivier Dumortier, Charlotte Hinault, and Emmanuel Van Obberghen
Cell Metabolism, 2013, Volume 18, Number 3, Page 312
Valeria Nesca, Claudiane Guay, Cécile Jacovetti, Véronique Menoud, Marie-Line Peyot, D. Ross Laybutt, Marc Prentki, and Romano Regazzi
Diabetologia, 2013, Volume 56, Number 10, Page 2203
Jonathan M. Locke, Hana Lango Allen, and Lorna W. Harries
Acta Diabetologica, 2014, Volume 51, Number 2, Page 325
Yiping Mao, Ramkumar Mohan, Shungang Zhang, and Xiaoqing Tang
Pharmacological Research, 2013, Volume 75, Page 37
Hyo-Jin Jeong, Seung-Yoon Park, Won-Mo Yang, and Wan Lee
Biochemical and Biophysical Research Communications, 2013, Volume 434, Number 3, Page 503
Cinzia Ciccacci, Davide Di Fusco, Laura Cacciotti, Roberto Morganti, Cinzia D’Amato, Carla Greco, Sara Rufini, Giuseppe Novelli, Federica Sangiuolo, Vincenza Spallone, and Paola Borgiani
Acta Diabetologica, 2013, Volume 50, Number 6, Page 867
Seung-Yoon Park, Hyo-Jin Jeong, Won-Mo Yang, and Wan Lee
Archives of Pharmacal Research, 2013, Volume 36, Number 2, Page 154
Xu Zhao, Qing Ye, Kang Xu, Jinluo Cheng, Yanqin Gao, Qian Li, Juan Du, Hui Shi, and Ling Zhou
Journal of Human Genetics, 2013, Volume 58, Number 3, Page 135
Guy W. Novotny, Morten Lundh, Marie B. Backe, Dan P. Christensen, Jakob B. Hansen, Mattias S. Dahllöf, Emil M.H. Pallesen, and Thomas Mandrup-Poulsen
Archives of Biochemistry and Biophysics, 2012, Volume 528, Number 2, Page 171
Cécile Jacovetti, Amar Abderrahmani, Géraldine Parnaud, Jean-Christophe Jonas, Marie-Line Peyot, Marion Cornu, Ross Laybutt, Emmanuelle Meugnier, Sophie Rome, Bernard Thorens, Marc Prentki, Domenico Bosco, and Romano Regazzi
Journal of Clinical Investigation, 2012, Volume 122, Number 10, Page 3541
Annika Bagge, Trine R. Clausen, Sylvester Larsen, Mette Ladefoged, Maiken W. Rosenstierne, Louise Larsen, Ole Vang, Jens H. Nielsen, and Louise T. Dalgaard
Biochemical and Biophysical Research Communications, 2012, Volume 426, Number 2, Page 266
C. Guay, C. Jacovetti, V. Nesca, A. Motterle, K. Tugay, and R. Regazzi
Diabetes, Obesity and Metabolism, 2012, Volume 14, Page 12
Michael D. Williams and Geraldine M. Mitchell
Experimental Diabetes Research, 2012, Volume 2012, Page 1
Dwi Setyowati Karolina, Arunmozhiarasi Armugam, Sugunavathi Sepramaniam, and Kandiah Jeyaseelan
Expert Review of Endocrinology & Metabolism, 2012, Volume 7, Number 3, Page 281
Nidhi Chaudhary, Kiran Kumar Nakka, Nilanjana Maulik, and Samit Chattopadhyay
Antioxidants & Redox Signaling, 2012, Volume 17, Number 2, Page 254
Veerle Rottiers and Anders M. Näär
Nature Reviews Molecular Cell Biology, 2012, Volume 13, Number 4, Page 239
S. Shantikumar, A. Caporali, and C. Emanueli
Cardiovascular Research, 2012, Volume 93, Number 4, Page 583
Mustafa Abdo Saif Dehwah, Aimin Xu, and Qingyang Huang
Journal of Genetics and Genomics, 2012, Volume 39, Number 1, Page 11
Nadine N. Baroukh and Emmanuel Van Obberghen
FEBS Journal, 2009, Volume 276, Number 22, Page 6509
Claudiane Guay, Elodie Roggli, Valeria Nesca, Cécile Jacovetti, and Romano Regazzi
Translational Research, 2011, Volume 157, Number 4, Page 253
Mayrin Correa-Medina, Valia Bravo-Egana, Samuel Rosero, Camillo Ricordi, Helena Edlund, Juan Diez, and Ricardo L. Pastori
Gene Expression Patterns, 2009, Volume 9, Number 4, Page 193
Galia Gat-Yablonski, Michal Yackobovitch-Gavan, and Moshe Phillip
Pediatric Clinics of North America, 2011, Volume 58, Number 5, Page 1117
Galia Gat-Yablonski, Michal Yackobovitch-Gavan, and Moshe Phillip
Endocrinology and Metabolism Clinics of North America, 2009, Volume 38, Number 3, Page 565
Nicholas Redshaw, Guy Wheeler, Mohammad K. Hajihosseini, and Tamas Dalmay
Brain Research, 2009, Volume 1250, Page 20
Erica Hennessy, Martin Clynes, Per Bendix Jeppesen, and Lorraine O’Driscoll
Biochemical and Biophysical Research Communications, 2010, Volume 396, Number 2, Page 457
Dethardt Müller, Hermann Katinger, and Johannes Grillari
Trends in Biotechnology, 2008, Volume 26, Number 7, Page 359
Francis C. Lynn
Trends in Endocrinology & Metabolism, 2009, Volume 20, Number 9, Page 452
Faer S Morrison, Karen A Johnstone, and Lorna W Harries
Expert Review of Endocrinology & Metabolism, 2011, Volume 6, Number 2, Page 255
Tal Melkman-Zehavi, Roni Oren, Sharon Kredo-Russo, Tirosh Shapira, Amitai D Mandelbaum, Natalia Rivkin, Tomer Nir, Kim A Lennox, Mark A Behlke, Yuval Dor, and Eran Hornstein
The EMBO Journal, 2011, Volume 30, Number 5, Page 835
Ryan Alexander, Harvey Lodish, and Lei Sun
Expert Opinion on Therapeutic Targets, 2011, Page 1
Maria Luisa S. Sequeira Lopez and R. Ariel Gomez
Current Hypertension Reports, 2010, Volume 12, Number 1, Page 26
Journal of Thrombosis and Haemostasis, 2010, Volume 8, Number 2, Page 369
M Garofalo, G L Condorelli, C M Croce, and G Condorelli
Cell Death and Differentiation, 2010, Volume 17, Number 2, Page 200
I. G. M. Kolfschoten, E. Roggli, V. Nesca, and R. Regazzi
Diabetes, Obesity and Metabolism, 2009, Volume 11, Page 118
Erica Hennessy and Lorraine O'Driscoll
Expert Reviews in Molecular Medicine, 2008, Volume 10

Comments (0)

Please log in or register to comment.