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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

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1437-4315
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Volume 389, Issue 4

Issues

Purification and characterization of natural Ara h 8, the Bet v 1 homologous allergen from peanut, provides a novel isoform

Susanne Riecken
  • 1Molecular and Clinical Allergology, Research Center Borstel, Parkallee 22, D-22457 Borstel, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Buko Lindner / Arnd Petersen
  • 3Molecular and Clinical Allergology, Research Center Borstel, Parkallee 22, D-22457 Borstel, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Uta Jappe
  • 4Department of Dermatology, University of Heidelberg, Voßstr. 2, D-69115 Heidelberg, Germany
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/ Wolf-Meinhard Becker
  • 5Molecular and Clinical Allergology, Research Center Borstel, Parkallee 22, D-22457 Borstel, Germany
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Published Online: 2008-03-27 | DOI: https://doi.org/10.1515/BC.2008.038

Abstract

The peanut allergen Ara h 8 is an important allergen for birch pollen allergic patients because of the cross-reactivity to the homologous Bet v 1. As the existence of Ara h 8 has been shown at the cDNA level so far (AY328088) and the allergen has indirectly been detected as natural protein, it was the aim of our study to identify natural Ara h 8 in peanut extract and to develop a purification strategy. This was achieved using a unique combination of purification steps, including optimized extraction conditions, size exclusion and ion exchange chromatography and treatment of the interfering contaminants with iodo-acetic acid. A characterization of the protein by microsequencing showed discrepancies to the deduced amino acid sequence of AY328088. For this reason, we cloned and expressed a new Ara h 8 isoform from cDNA (EU046325). This IgE-reactive protein corresponds to the results of microsequencing, ESI-FTICR-MS and trypsin fingerprinting analysis of the authentic and purified nAra h 8. Apart from the ultimate use of recombinant allergens for diagnostic procedures, there is also a scientific need for the natural counterpart, as it represents an excellent reference point by which to compare protein characteristics and to standardize diagnostic and therapeutic allergens.

Keywords: Ara h 8; peanut allergy; PR-10 protein; purification; recombinant expression; trypsin fingerprinting

About the article

Corresponding author


Received: 2007-10-10

Accepted: 2007-12-21

Published Online: 2008-03-27

Published in Print: 2008-04-01


Citation Information: Biological Chemistry, Volume 389, Issue 4, Pages 415–423, ISSN (Online) 14374315, ISSN (Print) 14316730, DOI: https://doi.org/10.1515/BC.2008.038.

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