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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
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Volume 389, Issue 4

Issues

Ribosome display and selection of human anti-CD22 scFvs derived from an acute lymphocytic leukemia patient

Achim Rothe
  • 1CSIRO Molecular and Health Technologies, 343 Royal Parade, Parkville, Victoria 3052, Australia and Department I of Internal Medicine, Laboratory of Immunotherapy, University Hospital Cologne, Joseph Stelzmann Str. 9, D-50931 Cologne, Germany
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/ Anne Nathanielsz
  • 2CSIRO Molecular and Health Technologies, 343 Royal Parade, Parkville, Victoria 3052, Australia
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/ Frank Oberhäuser
  • 3Department I of Internal Medicine, Laboratory of Immunotherapy, University Hospital Cologne, Joseph Stelzmann Str. 9, D-50931 Cologne, Germany
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/ Elke Pogge von Strandmann
  • 4Department I of Internal Medicine, Laboratory of Immunotherapy, University Hospital Cologne, Joseph Stelzmann Str. 9, D-50931 Cologne, Germany
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/ Andreas Engert
  • 5Department I of Internal Medicine, Laboratory of Immunotherapy, University Hospital Cologne, Joseph Stelzmann Str. 9, D-50931 Cologne, Germany
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/ Peter J. Hudson
  • 6CSIRO Molecular and Health Technologies, 343 Royal Parade, Parkville, Victoria 3052, Australia
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/ Barbara E. Power
  • 7CSIRO Molecular and Health Technologies, 343 Royal Parade, Parkville, Victoria 3052, Australia
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Published Online: 2008-03-27 | DOI: https://doi.org/10.1515/BC.2008.045

Abstract

Novel in vitro methods for the display of antibody libraries against disease-related antigens have led to the development of powerful protein-based biotherapeutics. Eukaryotic ternary ribosome complexes can be used to display human single chain antibodies (scFvs) to isolate specific binding reagents to these antigens. Here, we present the isolation of human scFv against the immunotherapeutic target antigen CD22 from a patient-derived human scFv library using ribosome display technology. The ribosome complexes were enriched against the extra-cellular domain of human CD22 conjugated to magnetic beads. Isolated constructs were further affinity-matured and specific binding activity was demonstrated by surface plasmon resonance and validated using in vitro cell assays. The isolated human anti-CD22 scFvs can provide a basis for the development of new immunotherapeutic strategies in CD22-expressing malignant diseases.

Keywords: antibody engineering; immunotherapy; in vitro ribosome display

About the article

Corresponding author


Received: 2007-10-28

Accepted: 2007-12-17

Published Online: 2008-03-27

Published in Print: 2008-04-01


Citation Information: Biological Chemistry, Volume 389, Issue 4, Pages 433–439, ISSN (Online) 14374315, ISSN (Print) 14316730, DOI: https://doi.org/10.1515/BC.2008.045.

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