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Licensed Unlicensed Requires Authentication Published by De Gruyter May 15, 2008

Structures and specificity of the human kallikrein-related peptidases KLK 4, 5, 6, and 7

  • Mekdes Debela , Nathalie Beaufort , Viktor Magdolen , Norman M. Schechter , Charles S. Craik , Manfred Schmitt , Wolfram Bode and Peter Goettig
From the journal Biological Chemistry

Abstract

Human kallikrein-related peptidases (KLKs) are (chymo)-trypsin-like serine proteinases that are expressed in a variety of tissues such as prostate, ovary, breast, testis, brain, and skin. Although their physiological functions have been only partly elucidated, many of the KLKs appear to be useful prognostic cancer markers, showing distinct correlations between their expression levels and different stages of cancer. Recent advances in the purification of ‘new type’ recombinant KLKs allowed solution of the crystal structures of KLK4, KLK5, KLK6, and KLK7. Along with these data, enzyme kinetic studies and extended substrate specificity profiling have led to an understanding of the non-prime-side substrate preferences of KLK4, 5, 6, and 7. The shape and polarity of the specificity pockets S1–S4 explain well their substrate preferences. KLK4, 5, and 6 exhibit trypsin-like specificity, with a strong preference for Arg at the P1 position of substrates. In contrast, KLK7 displays a unique chymotrypsin-like specificity for Tyr, which is also preferred at P2. All four KLKs show little specificity for P3 residues and have a tendency to accept hydrophobic residues at P4. Interestingly, for KLK4, 5, and 7 extended charged surface regions were observed that most likely serve as exosites for physiological substrates.


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Published Online: 2008-05-15
Published in Print: 2008-06-01

©2008 by Walter de Gruyter Berlin New York

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