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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

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Bone marrow-derived mesenchymal stem cell transplantation does not improve quality of muscle reinnervation or recovery of motor function after facial nerve transection in rats

Maria Grosheva1a / Orlando Guntinas-Lichius2a / Stephan Arnhold3 / Emmanouil Skouras4 / Stefanie Kuerten5 / Michael Streppel6 / Srebrina K. Angelova7 / Konstantin Wewetzer8 / Christine Radtke9 / Sarah A. Dunlop10 / Doychin N. Angelov11

1Department of Otorhinolaryngology, University of Cologne, D-50931 Cologne, Germany

2Department of Otorhinolaryngology, Friedrich-Schiller University Jena, D-07740 Jena, Germany

3Department of Veterinary Anatomy, Justus-Liebig-University, D-35392 Giessen, Germany

4Department of Trauma, Hand and Reconstructive Surgery, University of Cologne, D-50931 Cologne, Germany

5Department of Anatomy I, University of Cologne, D-50931 Cologne, Germany

6Department of Otorhinolaryngology, University of Cologne, D-50931 Cologne, Germany

7Department of Otorhinolaryngology, University of Cologne, D-50931 Cologne, Germany

8Center of Anatomy, Hannover Medical School, Carl-Neuberg-Strasse 1, D-30625 Hannover, Germany

9Department of Plastic, Hand and Reconstructive Surgery, Hannover Medical School, D-30625 Hannover, Germany

10School of Animal Biology and Western Australian Institute for Medical Research, The University of Western Australia, Crawley 6009, Australia

11Department of Anatomy I, University of Cologne, D-50931 Cologne, Germany

Corresponding author

Citation Information: Biological Chemistry. Volume 389, Issue 7, Pages 873–888, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2008.100, June 2008

Publication History

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Recently, we devised and validated a novel strategy in rats to improve the outcome of facial nerve reconstruction by daily manual stimulation of the target muscles. The treatment resulted in full recovery of facial movements (whisking), which was achieved by reducing the proportion of pathologically polyinnervated motor endplates. Here, we posed whether manual stimulation could also be beneficial after a surgical procedure potentially useful for treatment of large peripheral nerve defects, i.e., entubulation of the transected facial nerve in a conduit filled with suspension of isogeneic bone marrow-derived mesenchymal stem cells (BM-MSCs) in collagen. Compared to control treatment with collagen only, entubulation with BM-MSCs failed to decrease the extent of collateral axonal branching at the lesion site and did not improve functional recovery. Post-operative manual stimulation of vibrissal muscles also failed to promote a better recovery following entubulation with BM-MSCs. We suggest that BM-MSCs promote excessive trophic support for regenerating axons which, in turn, results in excessive collateral branching at the lesion site and extensive polyinnervation of the motor endplates. Furthermore, such deleterious effects cannot be overridden by manual stimulation. We conclude that entubulation with BM-MSCs is not beneficial for facial nerve repair.

Keywords: axotomy; bone marrow-derived mesenchymal stem cells; collateral branching; motoneuron; motor endplates; polyinnervation

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