Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2015: 2.710
Rank 142 out of 289 in category Biochemistry & Molecular Biology in the 2015 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

Online
ISSN
1437-4315
See all formats and pricing
Volume 389, Issue 7 (Jul 2008)

Issues

In vitro differentiation of reprogrammed murine somatic cells into hepatic precursor cells

Tobias Cantz
  • Present address: Junior Research Group Stem Cell Biology, Cluster-of-Excellence ‘REBIRTH’, Hannover Medical School, D-30625 Hannover, Germany
    1Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstr. 20, D-48149 Münster, Germany
/ Martina Bleidißel
  • 2Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstr. 20, D-48149 Münster, Germany
/ Martin Stehling
  • 3Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstr. 20, D-48149 Münster, Germany
/ Hans R. Schöler
  • 4Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstr. 20, D-48149 Münster, Germany
Published Online: 2008-06-06 | DOI: https://doi.org/10.1515/BC.2008.107

Abstract

Recently, a new approach to reprogram somatic cells into pluripotent stem cells was shown by fusion of somatic cells with embryonic stem (ES) cells, which results in a tetraploid karyotype. Normal hepatocytes are often polyploid, so we decided to investigate the differentiation potential of fusion hybrids into hepatic cells. We chose toxic milk mice (a model of Wilson's disease) and performed initial transplantation experiments using this potential cell therapy approach. Mononuclear bone marrow cells from Rosa26 mice were fused with OG2 (Oct4-GFP transgenic) ES cells. Unfused ES cells were eliminated by selection with G418 for OG2-Rosa26 hybrids and fusion-derived colonies could be subcloned. Using an endodermal differentiation protocol, hepatic precursor cells could be generated. After FACS depletion of contaminating Oct4-GFP-positive cells, the hepatic precursor cells were transplanted into immunosuppressed toxic milk mice by intrasplenic injection. However, five out of eight mice showed teratoma formation within 3–6 weeks after transplantation in the spleen and liver. In conclusion, a hepatic precursor cell type was achieved from mononuclear bone marrow cell-ES cell hybrids and preliminary transplantation experiments confirmed engraftment, but also showed teratoma formation, which needs to be excluded by using more stringent purification strategies.

Keywords: embryonic stem cells; endodermal differentiation; fusion; metabolic liver disorders; reprogramming

About the article

Corresponding author


Received: 2007-11-30

Accepted: 2008-04-23

Published Online: 2008-06-06

Published in Print: 2008-07-01


Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2008.107. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Nina Xie and Beisha Tang
Stem Cells International, 2016, Volume 2016, Page 1
[2]
Axel Schambach, Tobias Cantz, Christopher Baum, and Toni Cathomen
Expert Opinion on Biological Therapy, 2010, Volume 10, Number 7, Page 1089
[3]
Selina Möbus, Dakai Yang, Qinggong Yuan, Timo H.-W. Lüdtke, Asha Balakrishnan, Malte Sgodda, Bhavna Rani, Andreas Kispert, Marcos J. Araúzo-Bravo, Arndt Vogel, Michael P. Manns, Michael Ott, Tobias Cantz, and Amar Deep Sharma
Journal of Hepatology, 2015, Volume 62, Number 1, Page 101
[4]
Y. Kashiwakura, T. Ohmori, J. Mimuro, S. Madoiwa, M. Inoue, M. Hasegawa, K. Ozawa, and Y. Sakata
Haemophilia, 2014, Volume 20, Number 1, Page e40
[5]
Haesun Youn, Song-Hee Kim, Kyung Ah Choi, and Sungtae Kim
Journal of Cellular Biochemistry, 2013, Volume 114, Number 4, Page 920
[6]
Nils Pfaff, Nico Lachmann, Saskia Kohlscheen, Malte Sgodda, Marcos J. Araúzo-Bravo, Boris Greber, Wilfried Kues, Silke Glage, Christopher Baum, Heiner Niemann, Axel Schambach, Tobias Cantz, and Thomas Moritz
Stem Cells and Development, 2012, Volume 21, Number 5, Page 689
[7]
Liam J. Drew, Gregg W. Crabtree, Sander Markx, Kimberly L. Stark, Florence Chaverneff, Bin Xu, Jun Mukai, Karine Fenelon, Pei-Ken Hsu, Joseph A. Gogos, and Maria Karayiorgou
International Journal of Developmental Neuroscience, 2011, Volume 29, Number 3, Page 259
[8]
Reto Eggenschwiler, Komal Loya, Malte Sgodda, Francoise André, and Tobias Cantz
Stem Cells International, 2011, Volume 2011, Page 1
[9]
Jae-Woo Jang, Won-Young Lee, Jae-Ho Lee, Sung-Hwan Moon, Chang-Hoon Kim, and Hyung-Min Chung
Biochemical and Biophysical Research Communications, 2011, Volume 410, Number 2, Page 183
[10]
Krishanu Saha and Rudolf Jaenisch
Cell Stem Cell, 2009, Volume 5, Number 6, Page 584
[11]
Alexandra Rolletschek and Anna M. Wobus
Biological Chemistry, 2009, Volume 390, Number 9
[12]
Stefan Hübner and Athina Efthymiadis
Histochemistry and Cell Biology, 2011, Volume 135, Number 2, Page 111
[13]
S. N. Carvalho, D. C. Lira, G. P. Oliveira, A. A. Thole, A. C. Stumbo, C. E. Caetano, R. G. Marques, and L. Carvalho
Histochemistry and Cell Biology, 2010, Volume 134, Number 5, Page 493
[14]
Anjan Kumar Das and Rajarshi Pal
Journal of Tissue Engineering and Regenerative Medicine, 2010, Page n/a

Comments (0)

Please log in or register to comment.
Log in