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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

Online
ISSN
1437-4315
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Volume 390, Issue 12

Issues

Identification of collagen IV derived danger/alarm signals in insect immunity by nanoLC-FTICR MS

Boran Altincicek
  • Institute of Phytopathology and Applied Zoology, Justus Liebig University of Giessen, D-35392 Giessen, Germany
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/ Arton Berisha
  • Institute of Inorganic and Analytical Chemistry, Justus Liebig University of Giessen, D-35392 Giessen, Germany
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/ Krishnendu Mukherjee
  • Institute of Phytopathology and Applied Zoology, Justus Liebig University of Giessen, D-35392 Giessen, Germany
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/ Bernhard Spengler
  • Institute of Inorganic and Analytical Chemistry, Justus Liebig University of Giessen, D-35392 Giessen, Germany
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/ Andreas Römpp
  • Institute of Inorganic and Analytical Chemistry, Justus Liebig University of Giessen, D-35392 Giessen, Germany
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/ Andreas Vilcinskas
  • Institute of Phytopathology and Applied Zoology, Justus Liebig University of Giessen, D-35392 Giessen, Germany
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Published Online: 2009-08-10 | DOI: https://doi.org/10.1515/BC.2009.128

Abstract

The immune system can be stimulated by microbial molecules as well as by endogenously derived danger/alarm signals of host origin. Using the lepidopteran model insect Galleria mellonella, we recently discovered that fragments of collagen IV, resulting from hydrolysis by microbial metalloproteinases, represent danger/alarm signals in insects. Here, we characterized immune-stimulatory peptides generated by thermolysin-mediated degradation of collagen IV using nanospray ionization Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) after separation by nanoscale liquid chromatography (nanoLC). The combination of FTICR MS analysis and de novo peptide sequencing resulted in the identification of 38 specific collagen IV fragments of which several peptides included the integrin-binding motif RGD/E known from numerous mammalian immune-related proteins. Custom-synthesized peptides corresponding either to the presently identified collagen peptide GIRGEHyp or to a well-known integrin-binding RGD peptide (GRGDS) were injected into G. mellonella to determine their immune-stimulatory activities in vivo. Both peptides stimulated immune cells and systemically the expression of lysozyme and a specific inhibitor of microbial metalloproteinases. Further examination using specific MAP kinase inhibitors indicated that MEK/ERK and p38 are involved in RGD/E-mediated immune-signaling pathways, whereas JNK seems to play only a minor role.

Keywords: collagen; danger signals; Galleria mellonella; innate immunity; insect model organism; integrin

About the article

Corresponding author


Received: 2009-07-24

Accepted: 2009-07-29

Published Online: 2009-08-10

Published in Print: 2009-12-01


Citation Information: Biological Chemistry, Volume 390, Issue 12, Pages 1303–1311, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2009.128.

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