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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

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1437-4315
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Volume 390, Issue 3

Issues

The influence of linoleic and linolenic acid on the activity and intracellular localisation of phospholipase D in COS-1 cells

Anja Gemeinhardt
  • 1Institute of Biochemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, D-04103 Leipzig, Germany
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/ Marwan Alfalah
  • 2Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
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/ Thomas Gück
  • 3Institute of Biochemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, D-04103 Leipzig, Germany
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/ Hassan Y. Naim
  • 4Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Bünteweg 17, D-30559 Hannover, Germany
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/ Herbert Fuhrmann
  • 5Institute of Biochemistry, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 1, D-04103 Leipzig, Germany
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Published Online: 2008-12-17 | DOI: https://doi.org/10.1515/BC.2009.024

Abstract

Phospholipase D (PLD) is a receptor-regulated signalling enzyme involved in biological functions, such as exocytosis, phagocytosis, actin dynamics, membrane trafficking, and is considered to be essential for stimulated degranulation of cells. The purpose of our investigation was to examine how the fatty acid pattern of cellular membranes influences the activities and cellular distribution of the PLD1 and PLD2 isoforms. Expression of GFP-tagged PLD1 and PLD2 in COS-1 cells that were stimulated with mastoparan after cultivation in 20 μmol linoleic (C18:2n6) or linolenic (C18:3n3) acid for 4 d demonstrated that PLD1 dramatically alters its cellular distribution and is redistributed from intracellular vesicles to the cell surface. PLD2, on the other hand, maintains its localisation at the plasma membrane. The activity of PLD, which corresponds to PLD1 and PLD2, significantly increased two- to three-fold in the presence of the fatty acids. We conclude that linoleic acid and linolenic acid supplementation affect the intracellular trafficking of the PLD1 isoform and the activity of PLD most likely due to alterations in the membrane lipid environment conferred by the fatty acids.

Keywords: mastoparan; phospholipase D; polyunsaturated fatty acid; transphosphatidylation

About the article

Corresponding author


Received: 2008-08-17

Accepted: 2008-11-27

Published Online: 2008-12-17

Published in Print: 2009-03-01


Citation Information: Biological Chemistry, Volume 390, Issue 3, Pages 253–258, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2009.024.

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