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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
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1437-4315
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Volume 390, Issue 3

Issues

Regulation of phosphoenolpyruvate carboxylase in PVYNTN-infected tobacco plants

Karel Müller
  • 1Department of Biochemistry, Faculty of Natural Science, Charles University, Hlavova 2030, CZ-128 00 Prague 2, Czech Republic and Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-165 02 Prague 6, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Veronika Doubnerová
  • 2Department of Biochemistry, Faculty of Natural Science, Charles University, Hlavova 2030, CZ-128 00 Prague 2, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Helena Synková
  • 3Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-165 02 Prague 6, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Noemi Čeřovská
  • 4Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, CZ-165 02 Prague 6, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Helena Ryšlavá
  • 5Department of Biochemistry, Faculty of Natural Science, Charles University, Hlavova 2030, CZ-128 00 Prague 2, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2008-12-17 | DOI: https://doi.org/10.1515/BC.2009.029

Abstract

The effect of viral infection on the regulation of phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31) in Nicotiana tabacum L. leaves was studied. PEPC activity was 3 times higher in infected plant leaves compared to healthy plants. Activity of plant PEPC can be regulated, e.g., by de novo synthesis or reversible phosphorylation. The reason for the increase of PEPC activity as a consequence of PVYNTN infection was studied. The amount of PEPC determined by Western blot analysis or by relative estimation of PEPC mRNA by real-time PCR did not differ in control and PVYNTN-infected plants. Changes in post-translational modification of PEPC by phosphorylation were evaluated by comparing activity of the native and the dephosphorylated enzyme. The infected plants were characterized by a higher decrease of the enzyme activity after its dephosphorylation, which indicated a higher phosphorylation level. Immunochemical detection of phosphoproteins by Western blot analysis showed a more intensive band corresponding to PEPC from the infected material. This strengthens the hypothesis of an infection-related phosphorylation, which could be part of the plant's response to pathogen attack. The physiological implications of the increase in PEPC activity during PVYNTN infection are discussed.

Keywords: biotic stress; Nicotiana tabacum; phosphoenolpyruvate carboxylase (PEPC); phosphorylation

About the article

Corresponding author


Received: 2008-09-04

Accepted: 2008-12-02

Published Online: 2008-12-17

Published in Print: 2009-03-01


Citation Information: Biological Chemistry, Volume 390, Issue 3, Pages 245–251, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2009.029.

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