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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
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1437-4315
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Volume 390, Issue 3

Issues

Expression and localization of atypical PKC isoforms in liver parenchymal cells

Claudia Stross
  • 1Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich Heine University Düsseldorf, D-40225 Düsseldorf, Germany
  • Other articles by this author:
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/ Verena Keitel
  • 2Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich Heine University Düsseldorf, D-40225 Düsseldorf, Germany
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/ Elisabeth Winands
  • 3Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich Heine University Düsseldorf, D-40225 Düsseldorf, Germany
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/ Dieter Häussinger
  • 4Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich Heine University Düsseldorf, D-40225 Düsseldorf, Germany
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/ Ralf Kubitz
  • 5Clinic for Gastroenterology, Hepatology and Infectiology, Heinrich Heine University Düsseldorf, D-40225 Düsseldorf, Germany
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  • De Gruyter OnlineGoogle Scholar
Published Online: 2008-12-17 | DOI: https://doi.org/10.1515/BC.2009.031

Abstract

Members of all three classes of the protein kinase C (PKC) family including atypical PKCzeta (PKCζ) are involved in central functions of liver parenchymal cells. However, expression and localization of PKCiota (PKCι), the highly homologous atypical PKC (aPKC) isoform, in hepatocytes is unknown to date. PKCζ and PKCι were cloned from human and rat liver and fused to fluorescent protein tags (YFP). The sequence of full-length rat PKCι is not yet known and was cloned from cDNA of hepatocytes by the use of degenerated primers. PKCζ-YFP and PKCι-YFP (human and rat) were expressed in HeLa or HEK293 cells and used to test the specificity of seven aPKC antibodies. Two antibodies were PKCι-specific and two were specific for PKCζ in immunofluorescence and Western blot analysis. Subcellular localization was analyzed by immunofluorescence in isolated rat and human hepatocytes and liver sections. Low immunoreactivity for aPKCs was found at the sinusoidal membrane and in the cytosol. The highest density of PKCι as well as PKCζ was found at the canalicular membrane in co-localization with ABC-transporters, such as bile salt export pump or multidrug resistance-associated protein 2. This topology suggests a specific function of aPKCs at the canalicular membrane in addition to their known role in cell polarity of epithelial cells.

Keywords: antibodies; canalicular membrane; hepatocyte; rat PKCiota

About the article

Corresponding author


Received: 2008-08-21

Accepted: 2008-11-29

Published Online: 2008-12-17

Published in Print: 2009-03-01


Citation Information: Biological Chemistry, Volume 390, Issue 3, Pages 235–244, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2009.031.

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