Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year

IMPACT FACTOR 2015: 2.710
Rank 142 out of 289 in category Biochemistry & Molecular Biology in the 2015 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

See all formats and pricing
Volume 390, Issue 3 (Mar 2009)


The assembly and activation of kinin-forming systems on the surface of human U-937 macrophage-like cells

Anna Barbasz
  • 1Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland
/ Andrzej Kozik
  • 2Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland
Published Online: 2008-12-17 | DOI: https://doi.org/10.1515/BC.2009.032


A complex of three plasma proteins, including the high molecular mass kininogen (HK), prekallikrein (PK), and factor XII (FXII), is known to assemble on cell surfaces to release bradykinin-related proinflammatory peptides (kinins). Only recently, the binding of HK to human macrophages was described in the U-937 cell line model. In the present study, the adsorption of the other components of plasma kinin-generating system to these cells was characterized. FXII was found to tightly bind to U-937 cells and was also shown to partially compete with HK for the same binding sites on the macrophage surface. The Mac-1 and gC1qR proteins were found to be receptors for FXII on the cell surface. PK indirectly docked to the macrophages via the cell-bound HK and FXII. Within the complex of these proteins assembled on the macrophage, PK could be activated by FXII/FXIIa or independently of this factor, and the active PK effectively released kinins from HK. The cell surface-bound HK could also be the substrate for tissue kallikrein approaching the cell from the bulk fluid. The kinins released at the surface are suggested to induce secondary responses in the macrophages, leading to further propagation of the inflammatory state.

Keywords: bradykinin; contact system; factor XII; monocytes; plasma prekallikrein

About the article

Corresponding author

Received: 2008-09-20

Accepted: 2008-12-03

Published Online: 2008-12-17

Published in Print: 2009-03-01

Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2009.032. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Andrzej Kozik, Mariusz Gogol, Oliwia Bochenska, Justyna Karkowska-Kuleta, Natalia Wolak, Wojciech Kamysz, Wataru Aoki, Mitsuyoshi Ueda, Alexander Faussner, and Maria Rapala-Kozik
BMC Microbiology, 2015, Volume 15, Number 1, Page 60
Grazyna Bras, Oliwia Bochenska, Maria Rapala-Kozik, Ibeth Guevara-Lora, Alexander Faussner, Wojciech Kamysz, and Andrzej Kozik
Peptides, 2013, Volume 48, Page 114
Elena Kashuba, James Bailey, David Allsup, and Lynn Cawkwell
Biomarkers, 2013, Volume 18, Number 4, Page 279
Ibeth Guevara-Lora
Neurochemistry International, 2012, Volume 61, Number 1, Page 72
Ibeth Guevara-Lora, Magdalena Majkucinska, Anna Barbasz, Alexander Faussner, and Andrzej Kozik
Peptides, 2011, Volume 32, Number 6, Page 1193
Andrzej Kozik, Anna Golda, Pawel Mak, Piotr Suder, Jerzy Silberring, Anna Barbasz, and Maria Rapala-Kozik
Biological Chemistry, 2011, Volume 392, Number 3
Iwona Yike
Mycopathologia, 2011, Volume 171, Number 5, Page 299
Julius F. Varano della Vergiliana, Nithiananthan Asokananthan, and Geoffrey A. Stewart
Biological Chemistry, 2010, Volume 391, Number 9
J. S. Coelho dos Santos, C. A. S. Menezes, F. N. A. Villani, L. M. D. Magalhães, J. Scharfstein, K. J. Gollob, and W. O. Dutra
Clinical & Experimental Immunology, 2010, Volume 162, Number 3, Page 528

Comments (0)

Please log in or register to comment.
Log in