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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

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1437-4315
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Volume 390, Issue 4 (Apr 2009)

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Human CYP4Z1 catalyzes the in-chain hydroxylation of lauric acid and myristic acid

Andy Zöllner
  • 1PomBioTech GmbH, Campus Geb. A1-1, D-66123 Saarbrücken, Germany
/ Calin-Aurel Dragan
  • 2PomBioTech GmbH, Campus Geb. A1-1, D-66123 Saarbrücken, Germany
/ Dominik Pistorius
  • 3Department of Pharmaceutical Biotechnology, Saarland University, D-66041 Saarbrücken, Germany
/ Rolf Müller
  • 4Department of Pharmaceutical Biotechnology, Saarland University, D-66041 Saarbrücken, Germany
/ Helge B. Bode
  • 5Department of Pharmaceutical Biotechnology, Saarland University, D-66041 Saarbrücken, Germany
/ Frank T. Peters
  • 6Department of Experimental and Clinical Toxicology, Saarland University, D-66421 Homburg (Saar), Germany
/ Hans H. Maurer
  • 7Department of Experimental and Clinical Toxicology, Saarland University, D-66421 Homburg (Saar), Germany
/ Matthias Bureik
  • 8PomBioTech GmbH, Campus Geb. A1-1, D-66123 Saarbrücken, Germany
Published Online: 2008-12-17 | DOI: https://doi.org/10.1515/BC.2009.030

Abstract

Overexpression of human CYP4Z1, a cytochrome P450 enzyme, has been correlated with poor prognosis in human cancer. However, its catalytic properties are not yet known. We expressed this P450 in Schizosaccharomyces pombe and demonstrate by whole-cell biotransformation assays CYP4Z1-dependent in-chain hydroxylation of lauric and myristic acid, which in both cases leads to the formation of four different monohydroxylated products at positions ω-2, ω-3, ω-4, and ω-5, respectively. The CYP4Z1-expressing fission yeast should be a new valuable tool for testing cancer drugs or for the development of new prodrug strategies.

Keywords: breast cancer; cancer treatment; cytochrome P450; fatty acid hydroxylation; Schizosaccharomyces pombe; whole-cell biotransformation

About the article

Corresponding author


Received: 2008-11-21

Accepted: 2008-12-03

Published Online: 2008-12-17

Published in Print: 2009-04-01


Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2009.030.

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