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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2016: 1.679
Source Normalized Impact per Paper (SNIP) 2016: 0.800

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1437-4315
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Volume 390, Issue 4 (Apr 2009)

Issues

Staphylococcal cysteine protease staphopain B (SspB) induces rapid engulfment of human neutrophils and monocytes by macrophages

Jan Smagur
  • 1Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland
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/ Krzysztof Guzik
  • 2Department of Immunology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland
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/ Malgorzata Bzowska
  • 3Department of Immunology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland
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/ Mateusz Kuzak
  • 4Department of Cell Biophysics, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland
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/ Miroslaw Zarebski
  • 5Department of Cell Biophysics, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland
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/ Tomasz Kantyka
  • 6Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland
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/ Michal Walski
  • 7Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland
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/ Barbara Gajkowska
  • 8Department of Cell Ultrastructure, Mossakowski Medical Research Centre, Polish Academy of Sciences, 02-106 Warsaw, Poland
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/ Jan Potempa
  • 9Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-386 Krakow, Poland
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Published Online: 2009-03-05 | DOI: https://doi.org/10.1515/BC.2009.042

Abstract

Circulating neutrophils and monocytes constitute the first line of antibacterial defence, which is responsible for the phagocytosis and killing of microorganisms. Previously, we have described that the staphylococcal cysteine proteinase staphopain B (SspB) cleaves CD11b on peripheral blood phagocytes, inducing the rapid development of features of atypical cell death in protease-treated cells. Here, we report that exposure of phagocytes to SspB critically impairs their antibacterial functions. Specifically, SspB blocks phagocytosis of Staphylococcus aureus by both neutrophils and monocytes, represses their chemotactic activity and induces extensive, nonphlogistic clearance of SspB-treated cells by macrophages. The proteinase also cleaves CD31, a major repulsion (‘do not-eat-me’) signal, on the surface of neutrophils. We suggest that both proteolytic degradation of repulsion signals and induction of ‘eat-me’ signals on the surface of leukocytes are responsible for the observed intensive phagocytosis of SspB-treated neutrophils by human monocyte-derived macrophages. Collectively, this may lead to the depletion of functional neutrophils at the site of infection, thus facilitating staphylococcal colonisation and spreading.

Keywords: clearance; engulfment; infection; inflammation; phagocytosis; protease; receptor; Staphylococcus aureus

About the article

Corresponding author


Received: 2008-11-21

Accepted: 2009-01-31

Published Online: 2009-03-05

Published in Print: 2009-04-01


Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2009.042.

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