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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

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1437-4315
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Volume 391, Issue 1

Issues

Dexamethasone-dependent versus -independent markers of epithelial to mesenchymal transition in primary hepatocytes

Patricio Godoy
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Sumathi Lakkapamu
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Markus Schug
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Alexander Bauer
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Joanna D. Stewart
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Essam Bedawi
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Seddik Hammad
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Jakia Amin
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Rosemarie Marchan
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Wiebke Schormann
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Lindsey Maccoux
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Iris von Recklinghausen
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Raymond Reif
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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/ Jan G. Hengstler
  • Leibniz Research Center for Working Environment and Human Factors (IfADo), Ardeystrasse 67, D-44139 Dortmund, Germany
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Published Online: 2009-11-17 | DOI: https://doi.org/10.1515/bc.2010.010

Abstract

Recently, epithelial to mesenchymal transition (EMT) has been shown to represent a feature of dedifferentiating hepatocytes in vitro. Three-dimensional soft collagen gels can antagonize but not completely abolish this effect. Hormonal additives to culture media are known to maintain differentiated hepatocyte functions. Therefore, we studied whether insulin and dexamethasone antagonize EMT in cultured hepatocytes. Both hormones antagonized but not completely abolished certain morphological features of EMT. Dexamethasone antagonized acquisition of fibroblastoid shape, whereas insulin favored bile canaliculi formation. In a subsequent step, we analyzed expression of a battery of EMT-related genes. Of all markers tested, vimentin and snail-1 correlated best with morphological features of EMT. Interestingly, dexamethasone reduced expression levels of both vimentin and snail-1, whereas the influence of insulin was less pronounced. An important result of this study is that 12 out of 17 analyzed EMT markers were transcriptionally influenced by dexamethasone (vimentin, snail-1, snail-2, HNF4α, Twist-1, ZEB2, fibronectin, occludin, MMP14, claudin-1, cytokeratin-8, and cytokeratin-18), whereas the remaining factors seemed to be less dependent on dexamethasone. In conclusion, EMT markers in hepatocytes can be classified as dexamethasone-dependent versus -independent.

Keywords: dexamethasone; epithelial to mesenchymal transition; extracellular matrix; gene expression; hepatocytes; insulin

About the article

Corresponding author


Received: 2009-04-15

Accepted: 2009-09-24

Published Online: 2009-11-17

Published in Print: 2010-01-01


Citation Information: Biological Chemistry, Volume 391, Issue 1, Pages 73–83, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/bc.2010.010.

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