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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2015: 2.710
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Online
ISSN
1437-4315
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Volume 391, Issue 12 (Dec 2010)

Issues

Quantitative determination of haptoglobin glycoform variants in psoriasis

Bernardetta Maresca
  • Dipartimento di Patologia Sistematica – Sezione di Dermatologia, Università di Napoli Federico II, via Pansini 5, I-80131 Napoli, Italy
/ Luisa Cigliano
  • Dipartimento delle Scienze Biologiche, Università di Napoli Federico II, via Mezzocannone 8, I-80134 Napoli, Italy
/ Maria M. Corsaro
  • Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Complesso Universitario M.S. Angelo, via Cinthia 4, I-80126 Napoli, Italy
/ Giuseppina Pieretti
  • Dipartimento di Chimica Organica e Biochimica, Università di Napoli Federico II, Complesso Universitario M.S. Angelo, via Cinthia 4, I-80126 Napoli, Italy
/ Massimo Natale
  • BioDigitalValley Ltd, corso Vercelli 117, I-10015 Ivrea (Torino), Italy
/ Enrico M. Bucci
  • BioDigitalValley Ltd, corso Vercelli 117, I-10015 Ivrea (Torino), Italy
/ Fabrizio Dal Piaz
  • Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, via Ponte don Melillo, I-84084 Fisciano (Salerno), Italy
/ Nicola Balato
  • Dipartimento di Patologia Sistematica – Sezione di Dermatologia, Università di Napoli Federico II, via Pansini 5, I-80131 Napoli, Italy
/ Massimiliano Nino
  • Dipartimento di Patologia Sistematica – Sezione di Dermatologia, Università di Napoli Federico II, via Pansini 5, I-80131 Napoli, Italy
/ Fabio Ayala
  • Dipartimento di Patologia Sistematica – Sezione di Dermatologia, Università di Napoli Federico II, via Pansini 5, I-80131 Napoli, Italy
/ Paolo Abrescia
  • Dipartimento delle Scienze Biologiche, Università di Napoli Federico II, via Mezzocannone 8, I-80134 Napoli, Italy
Published Online: 2010-11-18 | DOI: https://doi.org/10.1515/bc.2010.146

Abstract

Haptoglobin is an acute phase glycoprotein, secreted by hepatocytes and other types of cells including keratinocytes. Haptoglobin has been suggested to impair the immune response, inhibit gelatinases in the extracellular matrix and promote angiogenesis, but its role in psoriasis is obscure to date. Changes in haptoglobin glycan structure were observed in several diseases. The aim of this study was to investigate whether haptoglobin displays glycan variations in psoriasis. We found that the pattern of plasma haptoglobin glycoforms, following two-dimensional electrophoresis, exhibited significant quantitative differences in spot intensities between patients and controls. Quantitative and qualitative differences in glycan mass, between patients and controls, were found by mass spectrometry of glycopeptides from tryptic digests of protein isolated from both patients and controls. The number of distinct fucosylated glycoforms of peptides NLFLNHSENATAK and MVSHHNLTTGATLINEQWLLTTAK was higher in patients than in controls, but no fucosylated glycan was detected on peptide VVLHPNYSQ-VDIGLIK in either case. The number of peptides with distinct triantennary and tetraantennary glycans was higher in patients than in controls. Abundance or structure of specific glycans, which are present in haptoglobin from patients and are different or missing in normal haptoglobin, might be associated with disease activity.

Keywords: glycans; mass spectrometry; structure heterogeneity; two-dimensional electrophoresis

About the article

Corresponding author


Received: 2010-07-27

Accepted: 2010-09-01

Published Online: 2010-11-18

Published in Print: 2010-12-01


Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/bc.2010.146. Export Citation

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