Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

Online
ISSN
1437-4315
See all formats and pricing
More options …
Just Accepted

Issues

Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation

Jitka Malcikova
  • Center of Molecular Biology and Gene Therapy, Department of Internal Medicine – Hematooncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Cernopolni 9, CZ-625 00 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Boris Tichy
  • Center of Molecular Biology and Gene Therapy, Department of Internal Medicine – Hematooncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Cernopolni 9, CZ-625 00 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jiri Damborsky
  • Loschmidt Laboratories, Institute of Experimental Biology and National Center for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5/A4, CZ-625 00 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jitka Kabathova
  • Center of Molecular Biology and Gene Therapy, Department of Internal Medicine – Hematooncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Cernopolni 9, CZ-625 00 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Martin Trbusek
  • Center of Molecular Biology and Gene Therapy, Department of Internal Medicine – Hematooncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Cernopolni 9, CZ-625 00 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jiri Mayer
  • Center of Molecular Biology and Gene Therapy, Department of Internal Medicine – Hematooncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Cernopolni 9, CZ-625 00 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sarka Pospisilova
  • Center of Molecular Biology and Gene Therapy, Department of Internal Medicine – Hematooncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Cernopolni 9, CZ-625 00 Brno, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2010-02-03 | DOI: https://doi.org/10.1515/bc.2010.027

Abstract

Sequence-specific DNA binding is the key function through which tumor suppressor p53 exerts transactivation of the downstream target genes, often being impaired in cancer cells by mutations in the TP53 gene. Functional protein microarray technology enables a high-throughput parallel analysis of protein properties within one experiment under the same conditions. Using an array approach, we analyzed the DNA binding activity of wild type p53 protein and of 49 variants. Our results show significant differences in the binding properties between the p53 mutants. The C-terminal mutant R337C displayed the highest DNA binding activity on the array. However, the same mutant showed only a partial activation in the reporter gene assay and almost no activation of downstream target genes after transfection of expression vector into cells lacking endogenous p53. These observations demonstrate that DNA binding itself is not sufficient for activating the p53 target genes in at least some of the p53 mutants and, therefore, in vitro studies might not always reflect in vivo conditions.

Keywords: R337C; real-time PCR; reporter gene assay; transactivation

About the article

Corresponding author


Received: 2009-07-03

Accepted: 2009-12-07

Published Online: 2010-02-03

Published in Print: 2010-01-01


Citation Information: Biological Chemistry, Volume 391, Issue 2/3, Pages 197–205, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/bc.2010.027.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Ana Gomes, Filipa Trovão, Benedita Andrade Pinheiro, Filipe Freire, Sara Gomes, Carla Oliveira, Lucília Domingues, Maria Romão, Lucília Saraiva, and Ana Carvalho
International Journal of Molecular Sciences, 2018, Volume 19, Number 4, Page 1184
[2]
María Ramos-Payán, Juan A. Ocaña-González, Rut M. Fernández-Torres, Santiago Maspoch, and Miguel Ángel Bello-López
Talanta, 2017, Volume 174, Page 738
[3]
Ting Tao, Hui Shi, Yihong Guan, Delai Huang, Ye Chen, David P Lane, Jun Chen, and Jinrong Peng
Cell Research, 2013, Volume 23, Number 5, Page 620
[4]
S Pospisilova, D Gonzalez, J Malcikova, M Trbusek, D Rossi, A P Kater, F Cymbalista, B Eichhorst, M Hallek, H Döhner, P Hillmen, M van Oers, J Gribben, P Ghia, E Montserrat, S Stilgenbauer, and T Zenz
Leukemia, 2012, Volume 26, Number 7, Page 1458

Comments (0)

Please log in or register to comment.
Log in