Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2015: 2.710
Rank 142 out of 289 in category Biochemistry & Molecular Biology in the 2015 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

Online
ISSN
1437-4315
See all formats and pricing
Volume 392, Issue 10 (Oct 2011)

Issues

Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells

Melanie Wurm
  • Institute for Transfusion Medicine, Hannover Medical School, D-30625 Hannover, Germany
  • Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
/ Benjamin Groß
  • Department of Paediatric Haematology and Oncology, Hannover Medical School, D-30625 Hannover, Germany
  • JRG Stem Cell Biology, Cluster of Excellence REBIRTH, Hannover Medical School, D-30625 Hannover, Germany
/ Malte Sgodda
  • JRG Stem Cell Biology, Cluster of Excellence REBIRTH, Hannover Medical School, D-30625 Hannover, Germany
/ Ludger Ständker
  • Department of Immunology and Rheumatology, Hannover Medical School, D-30625 Hannover, Germany
/ Thomas Müller
  • Institute for Transfusion Medicine, Hannover Medical School, D-30625 Hannover, Germany
/ Wolf-Georg Forssmann
  • Department of Immunology and Rheumatology, Hannover Medical School, D-30625 Hannover, Germany
/ Peter A. Horn
  • Institute for Transfusion Medicine, University Hospital Essen, D-45147 Essen, Germany
/ Rainer Blasczyk
  • Institute for Transfusion Medicine, Hannover Medical School, D-30625 Hannover, Germany
/ Tobias Cantz
  • JRG Stem Cell Biology, Cluster of Excellence REBIRTH, Hannover Medical School, D-30625 Hannover, Germany
  • Email:
Published Online: 2011-08-04 | DOI: https://doi.org/10.1515/BC.2011.085

Abstract

Genetic modification of human embryonic stem cells (hESCs) using biophysical DNA transfection methods are hampered by the very low single cell survival rate and cloning efficiency of hESCs. Lentiviral gene transfer strategies are widely used to genetically modify hESCs but limited transduction efficiencies in the presence of feeder or stroma cells present problems, particularly if vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped viral particles are applied. Here, we investigated whether the recently described semen derived enhancer of virus infection (SEVI) and alternative viral envelope proteins derived from either Gibbon ape leukaemia virus (GALV) or feline leukaemia virus (RD114) are applicable for transducing hESCs during co-culture with feeder or stroma cells. Our first set of experiments demonstrates that SEVI has no toxic effect on murine or hESCs and that exposure to SEVI does not interfere with the pluripotency-associated phenotype. Focusing on hESCs, we were able to further demonstrate that SEVI increases the transduction efficiencies of GALV and RD114 pseudotyped lentiviral vectors. More importantly, aiming at targeted differentiation of hESCs into functional somatic cell types, GALV pseudotyped lentiviral particles could efficiently and exclusively transduce hESCs grown in co-culture with OP9-GFP stroma cells (which were often used to induce differentiation into haematopoietic derivatives).

Keywords: embryonic stem cells; lentiviral gene transfer; SEVI; transduction efficiency

About the article

Corresponding author


Received: 2011-04-19

Accepted: 2011-06-27

Published Online: 2011-08-04

Published in Print: 2011-10-01


Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2011.085. Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Christoph Meier, Tanja Weil, Frank Kirchhoff, and Jan Münch
Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2014, Volume 6, Number 5, Page 438
[2]
Kristin Schwanke, Sylvia Merkert, Henning Kempf, Susann Hartung, Monica Jara-Avaca, Christian Templin, Gudrun Göhring, Axel Haverich, Ulrich Martin, and Robert Zweigerdt
Human Gene Therapy Methods, 2014, Volume 25, Number 2, Page 136
[3]
Maria Mercedes Segura, Mathias Mangion, Bruno Gaillet, and Alain Garnier
Expert Opinion on Biological Therapy, 2013, Volume 13, Number 7, Page 987

Comments (0)

Please log in or register to comment.
Log in