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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

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1437-4315
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Volume 392, Issue 11

Issues

Factor Xa subsite mapping by proteome-derived peptide libraries improved using WebPICS, a resource for proteomic identification of cleavage sites

Oliver Schilling / Ulrich auf dem Keller / Christopher M. Overall
  • Department of Biochemistry and Molecular Biology, Centre for Blood Research, University of British Columbia, Vancouver, Canada
  • Department of Oral Biological and Medical Sciences, Centre for Blood Research, University of British Columbia, Vancouver, Canada
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2011-08-17 | DOI: https://doi.org/10.1515/BC.2011.158

Abstract

Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based data-analysis resource termed WebPics (http://clipserve.clip.ubc.ca/pics/). We demonstrate the utility of WebPics analysis of PICS data by determining the substrate specificity of factor Xa from P6-P6’, an important blood coagulation protease that proteolytically generates thrombin from prothrombin. PICS confirms existing data on non-prime site specificity and refines our knowledge of factor Xa prime-site selectivity.

Keywords: peptide library; prime-site specificity; protease specificity

About the article

Corresponding author


Received: 2011-05-15

Accepted: 2011-08-04

Published Online: 2011-08-17

Published in Print: 2011-11-01


Citation Information: Biological Chemistry, Volume 392, Issue 11, Pages 1031–1037, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2011.158.

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