Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 392, Issue 5

Issues

Inhibition of AP-1 suppresses cervical cancer cell proliferation and is associated with p21 expression

Michelle F. Maritz
  • Division of Medical Biochemistry, Faculty of Health Sciences, University of Cape Town, Institute of Infectious Disease and Molecular Medicine, South Africa
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Pauline J. van der Watt
  • Division of Medical Biochemistry, Faculty of Health Sciences, University of Cape Town, Institute of Infectious Disease and Molecular Medicine, South Africa
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Nina Holderness
  • Division of Medical Biochemistry, Faculty of Health Sciences, University of Cape Town, Institute of Infectious Disease and Molecular Medicine, South Africa
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Michael J. Birrer
  • Harvard Medical School, Gynecologic Cancer Research Program, Gillette Center for Gynecologic Oncology, Massachusetts General Hospital, Boston, MA 02114, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Virna D. Leaner
  • Division of Medical Biochemistry, Faculty of Health Sciences, University of Cape Town, Institute of Infectious Disease and Molecular Medicine, South Africa
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2011-06-18 | DOI: https://doi.org/10.1515/bc.2011.036

Abstract

AP-1, a transcription factor comprised primarily of Jun and Fos family proteins, regulates genes involved in proliferation, differentiation and oncogenesis. Previous studies demonstrated that elevated expression of Jun and Fos family member proteins is associated with numerous human cancers and in cancer-relevant biological processes. In this study we used a dominant-negative mutant of c-Jun, Tam67, which interferes with the functional activity of all AP-1 complexes, to investigate the requirement of AP-1 in the proliferation and cell cycle progression of cervical cancer cells. Transient and stable expression of Tam67 in CaSki cervical cancer cells resulted in decreased AP-1 activity that correlated with a significant inhibition of cell proliferation and anchorage-independent colony formation. Inhibiting AP-1 activity resulted in a two-fold increase in cells located in the G2/M phase of the cell cycle and an accompanying increase in the expression of the cell cycle regulatory protein, p21. The increase in p21 was associated with a decrease in HPV E6 expression and an increase in p53. Importantly, blocking the induction of p21 in CaSki-Tam67-expressing cells accelerated their proliferation rate to that of CaSki, implicating p21 as a key player in the growth arrest induced by Tam67. Our results suggest a role for AP-1 in the proliferation, G2/M progression and inhibition of p21 expression in cervical cancer.

Keywords: AP-1; cervical cancer; p21; Tam67

About the article

Corresponding author


Received: 2010-06-25

Accepted: 2010-12-02

Published Online: 2011-06-18

Published in Print: 2011-05-01


Citation Information: Biological Chemistry, Volume 392, Issue 5, Pages 439–448, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/bc.2011.036.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Sarah Carden, Pauline van der Watt, Alicia Chi, Aderonke Ajayi-Smith, Katie Hadley, and Virna D. Leaner
BMC Cancer, 2018, Volume 18, Number 1
[3]
XIAO CHENG, HAOXUAN LUO, ZIJUN HOU, YAN HUANG, JINGBO SUN, and LIHUA ZHOU
Molecular Medicine Reports, 2014, Volume 10, Number 4, Page 1881
[4]
W.G. Jiang, A.J. Sanders, M. Katoh, H. Ungefroren, F. Gieseler, M. Prince, S.K. Thompson, M. Zollo, D. Spano, P. Dhawan, D. Sliva, P.R. Subbarayan, M. Sarkar, K. Honoki, H. Fujii, A.G. Georgakilas, A. Amedei, E. Niccolai, A. Amin, S.S. Ashraf, L. Ye, W.G. Helferich, X. Yang, C.S. Boosani, G. Guha, M.R. Ciriolo, K. Aquilano, S. Chen, A.S. Azmi, W.N. Keith, A. Bilsland, D. Bhakta, D. Halicka, S. Nowsheen, F. Pantano, and D. Santini
Seminars in Cancer Biology, 2015, Volume 35, Page S244
[5]
Rohan Rajapakse, Annesha Basu, Sanam Shahid, and Michael P. Timko
American Journal of Plant Sciences, 2014, Volume 05, Number 24, Page 3558
[6]
Shegan Gao, Jonathan Brown, Huizhi Wang, and Xiaoshan Feng
Archivum Immunologiae et Therapiae Experimentalis, 2014, Volume 62, Number 2, Page 131
[7]
Yan Ma, Jing Tian, Han Cen, Jing Li, Wang-Dong Xu, De-Guang Wang, Hai-Feng Pan, and Dong-Qing Ye
DNA and Cell Biology, 2012, Volume 31, Number 7, Page 1274

Comments (0)

Please log in or register to comment.
Log in