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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

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IMPACT FACTOR 2017: 3.022

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1437-4315
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Volume 392, Issue 8-9

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Characterization of presynaptic septin complexes in mammalian hippocampal neurons

Christopher W. Tsang
  • Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, M5S 1AB, Ontario, Canada
  • Program in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, M5G 1XB, Ontario, Canada
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mathew P. Estey
  • Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, M5S 1AB, Ontario, Canada
  • Program in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, M5G 1XB, Ontario, Canada
  • Other articles by this author:
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/ Jessica E. DiCiccio
  • Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, M5S 1AB, Ontario, Canada
  • Program in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, M5G 1XB, Ontario, Canada
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Hong Xie
  • Program in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, M5G 1XB, Ontario, Canada
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Dana Patterson
  • Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, M5S 1AB, Ontario, Canada
  • Program in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, M5G 1XB, Ontario, Canada
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ William S. Trimble
  • Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, M5S 1AB, Ontario, Canada
  • Program in Cell Biology, Hospital for Sick Children, 555 University Avenue, Toronto, M5G 1XB, Ontario, Canada
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  • Other articles by this author:
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Abstract

Septins are GTPases that form heteromeric complexes and are linked to neurological disorders. Although several septin subcomplexes have been reported in various mammalian tissues, the cellular and subcellular distribution of these complexes is largely unexplored. Using antibodies against ten mammalian septins, we show that septins diverge with respect to their tissue distribution implying that septin complexes in various tissues have unique composition. Although all ten septins examined were expressed in brain tissue, we describe septin complex(es) including SEPT3, SEPT5, SEPT6, SEPT7 and SEPT11 that could be functional within the presynapse because, unlike other septins they: (1) showed an increase in expression from embryonic day 15 to post-natal day 70, (2) were abundantly expressed in axons and growth cones of developing hippocampal neurons, (3) were found in presynaptic terminals of mature synapses, (4) were enriched in a preparation of synaptic vesicles and (5) immunoprecipitated together from brain tissue and cultured nerve cells. Knockdown of SEPT5 or SEPT7 in developing hippocampal neurons impaired axon growth. Because septins are functionally linked to the cytoskeleton and vesicle traffic, presynaptic neuronal septin complexes could be important for ensuring proper axon development and neurotransmitter release.

Keywords: exocytosis; GTPase; nerve terminal; neurite; polarity; synaptic vesicle

About the article

Corresponding author


Received: 2011-04-11

Accepted: 2011-06-10

Published in Print: 2011-08-01


Citation Information: Biological Chemistry, Volume 392, Issue 8-9, Pages 739–749, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2011.077.

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