Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2016: 3.273

CiteScore 2016: 3.01

SCImago Journal Rank (SJR) 2016: 1.679
Source Normalized Impact per Paper (SNIP) 2016: 0.800

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 392, Issue 8-9 (Aug 2011)

Issues

Evidence that a septin diffusion barrier is dispensable for cytokinesis in budding yeast

Carsten Wloka
  • Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA
  • Institute of Chemistry and Biochemistry, Department of Membrane Biochemistry, Freie Universität Berlin, Takustraße 6, D-14195 Berlin, Germany
  • These authors contributed equally to this work.
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Ryuichi Nishihama
  • Department of Genetics, Stanford University of School of Medicine, Stanford, CA 94305, USA
  • These authors contributed equally to this work.
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Masayuki Onishi / Younghoon Oh
  • Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Julia Hanna
  • Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ John R. Pringle / Michael Krauß
  • Institute of Chemistry and Biochemistry, Department of Membrane Biochemistry, Freie Universität Berlin, Takustraße 6, D-14195 Berlin, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Erfei Bi
  • Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6058, USA
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar

Abstract

Septins are essential for cytokinesis in Saccharomyces cerevisiae, but their precise roles remain elusive. Currently, it is thought that before cytokinesis, the hourglass-shaped septin structure at the mother-bud neck acts as a scaffold for assembly of the actomyosin ring (AMR) and other cytokinesis factors. At the onset of cytokinesis, the septin hourglass splits to form a double ring that sandwiches the AMR and may function as diffusion barriers to restrict diffusible cytokinesis factors to the division site. Here, we show that in cells lacking the septin Cdc10 or the septin-associated protein Bud4, the septins form a ring-like structure at the mother-bud neck that fails to re-arrange into a double ring early in cytokinesis. Strikingly, AMR assembly and constriction, the localization of membrane-trafficking and extracellular-matrix-remodeling factors, cytokinesis, and cell-wall-septum formation all occur efficiently in cdc10Δ and bud4Δ mutants. Thus, diffusion barriers formed by the septin double ring do not appear to be critical for S. cerevisiae cytokinesis. However, an AMR mutation and a septin mutation have synergistic effects on cytokinesis and the localization of cytokinesis proteins, suggesting that tethering to the AMR and a septin diffusion barrier may function redundantly to localize proteins to the division site.

Keywords: Bud4; chitin synthase; exocyst; membrane-trafficking; septum formation; W303

About the article

Corresponding author


Received: 2011-05-08

Accepted: 2011-06-22

Published in Print: 2011-08-01


Citation Information: Biological Chemistry, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2011.083.

Export Citation

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Andrew A. Bridges and Amy S. Gladfelter
Journal of Biological Chemistry, 2015, Volume 290, Number 28, Page 17173
[2]
Molly McQuilken, Maximilian S. Jentzsch, Amitabh Verma, Shalin B. Mehta, Rudolf Oldenbourg, and Amy S. Gladfelter
Frontiers in Cell and Developmental Biology, 2017, Volume 5
[3]
Yogini P. Bhavsar-Jog and Erfei Bi
Seminars in Cell & Developmental Biology, 2017, Volume 66, Page 107
[4]
Minami Orii, Keiko Kono, Hsin-I Wen, Makoto Nakanishi, and Takashi Toda
PLOS ONE, 2016, Volume 11, Number 1, Page e0146941
[5]
Christian Renz, Silke Oeljeklaus, Sören Grinhagens, Bettina Warscheid, Nils Johnsson, Thomas Gronemeyer, and Amy S. Gladfelter
PLOS ONE, 2016, Volume 11, Number 2, Page e0148340
[6]
Adam M. Perez, Gregory C. Finnigan, Françoise M. Roelants, and Jeremy Thorner
Frontiers in Cell and Developmental Biology, 2016, Volume 4
[7]
P.-L. Yang, T.-H. Hsu, C.-W. Wang, and R.-H. Chen
Molecular Biology of the Cell, 2016, Volume 27, Number 15, Page 2368
[8]
L. R. Heasley and M. A. McMurray
Molecular Biology of the Cell, 2016, Volume 27, Number 3, Page 442
[9]
Shawn N. Jordan, Tim Davies, Yelena Zhuravlev, Julien Dumont, Mimi Shirasu-Hiza, and Julie C. Canman
The Journal of Cell Biology, 2016, Volume 212, Number 1, Page 39
[10]
Lei Chen, Yu-Hang Zhang, Tao Huang, and Yu-Dong Cai
Molecular Genetics and Genomics, 2016, Volume 291, Number 2, Page 913
[11]
Anum Khan, Molly McQuilken, and Amy S. Gladfelter
Annual Review of Microbiology, 2015, Volume 69, Number 1, Page 487
[12]
L. Merlini, A. Bolognesi, M. A. Juanes, F. Vandermoere, T. Courtellemont, R. Pascolutti, M. Seveno, Y. Barral, and S. Piatti
Molecular Biology of the Cell, 2015, Volume 26, Number 18, Page 3245
[13]
Z. Feng, S. Okada, G. Cai, B. Zhou, and E. Bi
Molecular Biology of the Cell, 2015, Volume 26, Number 7, Page 1211
[14]
Katy Ong, Carsten Wloka, Satoshi Okada, Tatyana Svitkina, and Erfei Bi
Nature Communications, 2014, Volume 5, Page 5698
[15]
Corinne Cassani, Erica Raspelli, Elena Chiroli, and Roberta Fraschini
Cell Cycle, 2014, Volume 13, Number 10, Page 1590
[16]
Masayuki Onishi, Nolan Ko, Ryuichi Nishihama, and John R. Pringle
The Journal of Cell Biology, 2013, Volume 202, Number 2, Page 311
[17]
Nabila Founounou, Nicolas Loyer, and Roland Le Borgne
Developmental Cell, 2013, Volume 24, Number 3, Page 242
[18]
Carsten Wloka, Elizabeth A. Vallen, Lydia Thé, Xiaodong Fang, Younghoon Oh, and Erfei Bi
The Journal of Cell Biology, 2013, Volume 200, Number 3, Page 271
[19]
Carsten Wloka and Erfei Bi
Cytoskeleton, 2012, Volume 69, Number 10, Page 710
[20]
Serge Mostowy and Pascale Cossart
Nature Reviews Molecular Cell Biology, 2012
[21]
Alfred Wittinghofer
Biological Chemistry, 2011, Volume 392, Number 8-9

Comments (0)

Please log in or register to comment.
Log in