Jump to ContentJump to Main Navigation
Show Summary Details

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board Member: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2015: 2.710
Rank 142 out of 289 in category Biochemistry & Molecular Biology in the 2015 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 1.607
Source Normalized Impact per Paper (SNIP) 2015: 0.751
Impact per Publication (IPP) 2015: 2.609

Online
ISSN
1437-4315
See all formats and pricing

 


 
 

Select Volume and Issue

Issues

30,00 € / $42.00 / £23.00

Get Access to Full Text

N-terminal acetylation of annexin A2 is required for S100A10 binding

Ali Reza Nazmi
  • Institute of Medical Biochemistry, ZMBE, University of Münster, von-Esmarch-Str. 56, D-48149 Münster, Germany
  • These authors contributed equally to this work.
/ Gabriel Ozorowski
  • Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA
  • These authors contributed equally to this work.
/ Milena Pejic
  • Institute of Medical Biochemistry, ZMBE, University of Münster, von-Esmarch-Str. 56, D-48149 Münster, Germany
/ Julian P. Whitelegge
  • The Pasarow Mass Spectrometry Laboratory, The NPI-Semel Institute, David Geffen School of Medicine, University of California, Los Angeles, CA 90024, USA
/ Volker Gerke
  • Institute of Medical Biochemistry, ZMBE, University of Münster, von-Esmarch-Str. 56, D-48149 Münster, Germany
  • Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA
  • :
/ Hartmut Luecke
  • Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA
  • Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA
  • Department of Computer Science, University of California, Irvine, CA 92697, USA
  • Center for Biomembrane Systems, University of California, Irvine, CA
  • :
Published Online: 2012-09-08 | DOI: https://doi.org/10.1515/hsz-2012-0179

Abstract

Annexin A2 (AnxA2), a Ca2+-regulated phospholipid binding protein involved in membrane-cytoskeleton contacts and membrane transport, exists in two physical states, as a monomer or in a heterotetrameric complex mediated by S100A10. Formation of the AnxA2-S100A10 complex is of crucial regulatory importance because only the complex is firmly anchored in the plasma membrane, where it functions in the plasma membrane targeting/recruitment of certain ion channels and receptors. The S100A10 binding motif is located in the first 12 residues of the AnxA2 N-terminal domain, but conflicting reports exist as to the importance of N-terminal AnxA2 acetylation with regard to S100A10 binding. We show here that AnxA2 is subject to N-terminal modification when expressed heterologously in Escherichia coli. Met1 is removed and Ser2 is acetylated, yielding the same modification as the authentic mammalian protein. Bacterially expressed and N-terminally acetylated AnxA2 binds S100A10 with an affinity comparable to AnxA2 from porcine tissue and is capable of forming the AnxA2-S100A10 heterotetramer. Complex formation is competitively inhibited by acetylated but not by non-acetylated peptides covering the N-terminal AnxA2 sequence. These results demonstrate that N-terminal acetylation of AnxA2 is required for S100A10 binding and that this common eukaryotic modification is also obtained upon expression in bacteria.

Keywords: annexin; calcium; membrane binding; prokaryotic post-translational modification; protein interaction; Robert Huber

Corresponding authors: Volker Gerke and Hartmut Luecke, Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697-3900, USA


Received: 2012-04-16

Accepted: 2012-07-12

Published Online: 2012-09-08

Published in Print: 2012-10-01


Citation Information: . Volume 393, Issue 10, Pages 1141–1150, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/hsz-2012-0179, September 2012

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Max J. Dörfel and Gholson J. Lyon
Gene, 2015, Volume 567, Number 2, Page 103
[2]
Yidong Liu, Helene K Myrvang, and Lodewijk V Dekker
British Journal of Pharmacology, 2015, Volume 172, Number 7, Page 1664
[3]
Patrick Drücker, Milena Pejic, David Grill, Hans-Joachim Galla, and Volker Gerke
Biophysical Journal, 2014, Volume 107, Number 9, Page 2070
[4]
Gabriel Ozorowski, Saskia Milton, and Hartmut Luecke
Acta Crystallographica Section D Biological Crystallography, 2013, Volume 69, Number 1, Page 92

Comments (0)

Please log in or register to comment.