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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

12 Issues per year


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

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1437-4315
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Volume 393, Issue 11

Issues

The sensor kinase DcuS of Escherichia coli: two stimulus input sites and a merged signal pathway in the DctA/DcuS sensor unit

Julian Witan
  • Institute for Microbiology and Wine Research, Johannes Gutenberg University, D-55099 Mainz, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Christian Monzel
  • Institute for Microbiology and Wine Research, Johannes Gutenberg University, D-55099 Mainz, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Patrick D. Scheu
  • Institute for Microbiology and Wine Research, Johannes Gutenberg University, D-55099 Mainz, Germany
  • Present address: Centre for Bacterial Cell Biology, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gottfried Unden
  • Corresponding author
  • Institute for Microbiology and Wine Research, Johannes Gutenberg University, D-55099 Mainz, Germany
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2012-10-11 | DOI: https://doi.org/10.1515/hsz-2012-0229

Abstract

The membrane-integral sensor kinase DcuS of Escherichia coli consists of a periplasmically located sensory PASP domain, transmembrane helices TM1 and TM2, a cytoplasmic PASC domain and the kinase domain. Stimulus (C4-dicarboxylate) binding at PASP is required to stimulate phosphorylation of the kinase domain, resulting in phosphoryl transfer to the response regulator DcuR. PASC functions as a signaling device or a relay in signal transfer from TM2 to the kinase. Phosphorylated DcuR induces the expression of the target genes. Sensing by DcuS requires the presence of the C4-dicarboxylate transporter DctA during aerobic growth. DctA forms a sensor unit with DcuS, and a short C-terminal sequence of DctA forming the putative helix 8b is required for interaction with DcuS. Helix 8b contains a LDXXXLXXXL motif that is essential for function and interaction. DcuS requires the PASC domain for signal perception from DctA. Thus, DcuS and DctA form a DctA/DcuS sensory unit, and DcuS perceives stimuli from two different sites (PASP and DctA). The signal transfer pathways are supposed to merge at PASC. The fumarate/succinate antiporter DcuB takes over the role as a co-sensor of DcuS under anaerobic growth conditions.

Keywords: DctA; DcuS; PAS domain; sensing and signal transduction; sensor kinase

About the article

Corresponding author: Gottfried Unden, Institute for Microbiology and Wine Research, Johannes Gutenberg University, D-55099 Mainz, Germany


Received: 2012-06-21

Accepted: 2012-08-07

Published Online: 2012-10-11

Published in Print: 2012-11-01


Citation Information: , Volume 393, Issue 11, Pages 1291–1297, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/hsz-2012-0229.

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©2012 by Walter de Gruyter Berlin Boston.Get Permission

Citing Articles

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[1]
S. Graf, D. Schmieden, K. Tschauner, S. Hunke, and G. Unden
Journal of Bacteriology, 2014, Volume 196, Number 5, Page 1084
[2]
Patrick D. Scheu, Philipp A. Steinmetz, Felix Dempwolff, Peter L. Graumann, Gottfried Unden, and Hendrik W. van Veen
PLoS ONE, 2014, Volume 9, Number 12, Page e115534
[3]
Eduardo A. Groisman
Annual Review of Microbiology, 2016, Volume 70, Number 1, Page 103
[4]
Sebastian Wörner, Alexander Strecker, Christian Monzel, Matthias Zeltner, Julian Witan, Andrea Ebert-Jung, and Gottfried Unden
Environmental Microbiology, 2016, Volume 18, Number 12, Page 4920
[5]
Philipp Aloysius Steinmetz, Sebastian Wörner, and Gottfried Unden
Molecular Microbiology, 2014, Volume 94, Number 1, Page 218
[6]
[7]
Stephanie Nilkens, Mareike Koch-Singenstreu, Volker Niemann, Friedrich Götz, Thilo Stehle, and Gottfried Unden
Molecular Microbiology, 2014, Volume 91, Number 2, Page 381
[8]
Christian Monzel, Pia Degreif-Dünnwald, Christina Gröpper, Christian Griesinger, and Gottfried Unden
MicrobiologyOpen, 2013, Volume 2, Number 6, Page 912

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