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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

Online
ISSN
1437-4315
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Volume 393, Issue 3

Issues

Development of a host blood meal database: de novo sequencing of hemoglobin from nine small mammals using mass spectrometry

Ünige A. Laskay / Jennifer Burg / Erin J. Kaleta / Inger-Marie E. Vilcins
  • Departments of Microbiology and Molecular Genetics and Medicine, University of California Irvine, Irvine, CA 92697, USA
  • Present address: Vector-Borne Disease Section, California Department of Public Health, Ontario, CA, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sam R. Telford III
  • Tufts University, Cummings School of Veterinary Medicine, Division of Infectious Diseases, North Grafton, MA 01536, USA
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Alan G. Barbour
  • Departments of Microbiology and Molecular Genetics and Medicine, University of California Irvine, Irvine, CA 92697, USA
  • Other articles by this author:
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/ Vicki H. Wysocki

Abstract

We report the successful de novo sequencing of hemoglobin using a mass spectrometry-based approach combined with automatic data processing and manual validation for nine North American species with currently unsequenced genomes. The complete α and β chain of all nine mammalian hemoglobin samples used in this study were successfully sequenced. These sequences will be appended to the existing database containing all known hemoglobins to be used for identification of the mammalian host species that provided the last blood meal for the tick vector of Lyme disease, Ixodes scapularis.

Keywords: LC-MS/MS; PepNovo; species identification; tick-borne diseases

About the article

Corresponding author


Received: 2011-08-12

Accepted: 2011-12-24

Published in Print: 2012-03-01


Citation Information: , Volume 393, Issue 3, Pages 195–201, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/hsz-2011-0196.

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