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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

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1437-4315
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Volume 393, Issue 7

Issues

Biological and molecular analysis of the pathogenic variant C3 of potato spindle tuber viroid (PSTVd) evolved during adaptation to chamomile (Matricaria chamomilla)

Jaroslav Matoušek
  • BC ASCR v.v.i., Institute of Plant Molecular Biology, Branišovská 31, CZ-37005 České Budějovice, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jan Stehlík
  • BC ASCR v.v.i., Institute of Plant Molecular Biology, Branišovská 31, CZ-37005 České Budějovice, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Jitka Procházková
  • BC ASCR v.v.i., Institute of Plant Molecular Biology, Branišovská 31, CZ-37005 České Budějovice, Czech Republic
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Lidmila Orctová
  • BC ASCR v.v.i., Institute of Plant Molecular Biology, Branišovská 31, CZ-37005 České Budějovice, Czech Republic
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  • De Gruyter OnlineGoogle Scholar
/ Julia Wullenweber
  • Institute of Physical Biology, Heinrich Heine University Düsseldorf, D-40204 Düsseldorf, Germany
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/ Zoltan Füssy
  • Faculty of Science, University of South Bohemia, Branišovská 31, CZ-37005 České Budějovice, Czech Republic
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/ Josef Kováčik
  • Department of Botany, Faculty of Science, P. J. Šafárik University, Mánesova 23, 041 67 Košice, Slovak Republic
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  • De Gruyter OnlineGoogle Scholar
/ Ganesh S. Duraisamy
  • BC ASCR v.v.i., Institute of Plant Molecular Biology, Branišovská 31, CZ-37005 České Budějovice, Czech Republic
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  • De Gruyter OnlineGoogle Scholar
/ Angelika Ziegler
  • Institute for Biosafety of Genetically Modified Plants, Julius Kühn-Institute, Erwin-Baur-Str. 27, D-06484 Quedlinburg, Germany
  • Other articles by this author:
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/ Jörg Schubert
  • Institute for Biosafety of Genetically Modified Plants, Julius Kühn-Institute, Erwin-Baur-Str. 27, D-06484 Quedlinburg, Germany
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/ Gerhard Steger
  • Corresponding author
  • Institute of Physical Biology, Heinrich Heine University Düsseldorf, D-40204 Düsseldorf, Germany
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Abstract

Viroid-caused pathogenesis is a specific process dependent on viroid and host genotype(s), and may involve viroid-specific small RNAs (vsRNAs). We describe a new PSTVd variant C3, evolved through sequence adaptation to the host chamomile (Matricaria chamomilla) after biolistic inoculation with PSTVd-KF440-2, which causes extraordinary strong (‘lethal’) symptoms. The deletion of a single adenine A in the oligoA stretch of the pathogenicity (P) domain appears characteristic of PSTVd-C3. The pathogenicity and the vsRNA pool of PSTVd-C3 were compared to those of lethal variant PSTVd-AS1, from which PSTVd-C3 differs by five mutations located in the P domain. Both lethal viroid variants showed higher stability and lower variation in analyzed vsRNA pools than the mild PSTVd-QFA. PSTVd-C3 and -AS1 caused similar symptoms on chamomile, tomato, and Nicotiana benthamiana, and exhibited similar but species-specific distributions of selected vsRNAs as quantified using TaqMan probes. Both lethal PSTVd variants block biosynthesis of lignin in roots of cultured chamomile and tomato. Four ‘expression markers’ (TCP3, CIPK, VSF-1, and VPE) were selected from a tomato EST library to quantify their expression upon viroid infection; these markers were strongly downregulated in tomato leaf blades infected by PSTVd-C3- and -AS1 but not by PSTVd-QFA.

This article offers supplementary material which is provided at the end of the article.

Keywords: biolistic inoculation of plants; micro RNA probes; real-time PCR; viroid adaptation; viroid pathogenesis markers; viroid-specific small RNA profiles

About the article

Corresponding author


Received: 2011-12-15

Accepted: 2012-04-14

Published in Print: 2012-07-01


Citation Information: , Volume 393, Issue 7, Pages 605–615, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/hsz-2011-0286.

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