Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2017: 3.022

CiteScore 2017: 2.81

SCImago Journal Rank (SJR) 2017: 1.562
Source Normalized Impact per Paper (SNIP) 2017: 0.705

Online
ISSN
1437-4315
See all formats and pricing
More options …
Volume 393, Issue 8

Issues

Protein O-glycosylation analysis

Gerhild Zauner
  • Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, NL-2300 RC Leiden, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Radoslaw P. Kozak / Richard A. Gardner / Daryl L. Fernandes / André M. Deelder
  • Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, NL-2300 RC Leiden, The Netherlands
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Manfred Wuhrer
  • Corresponding author
  • Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, NL-2300 RC Leiden, The Netherlands
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar

Abstract

This review provides an overview on the methods available for analysis of O-glycosylation. Three major themes are addressed: analysis of released O-glycans including different O-glycan liberation, derivatization, and detection methods; analysis of formerly O-glycosylated peptides yielding information on O-glycan attachment sites; analysis of O-glycopeptides, representing by far the most informative but also most challenging approach for O-glycan analysis. Although there are various techniques available for the identification of O-linked oligosaccharides, the focus here is on MS fragmentation techniques such as collision-induced fragmentation, electron capture dissociation, and electron transfer dissociation. Finally, the O-glycan analytical challenges that need to be met will be discussed.

Keywords: electron capture dissociation; electron transfer dissociation; mass spectrometry; O-glycan; O-glycopeptide; O-glycosylation

About the article

Gerhild Zauner

Dr. Gerhild Zauner studied pharmaceutical sciences at the University of Graz (Austria) and the University of East Anglia (UK) and obtained her degree in 2004 before moving to Leiden (The Netherlands) for her PhD in Protein Chemistry/Biophysics at the Chemistry Institute under the supervision of Prof. Gerard W. Canters and Prof. Thijs J. Aartsma. At the beginning of 2009 she started a postdoc at the LUMC in Leiden in the group of Dr. Manfred Wuhrer in the fi eld of glycosylation analysis. Gerhild especially focuses on O-glycosylation analysis, and – together with industrial collaborations – specializes on the development of highthroughput methods for protein glycosylation analysis.

Radoslaw P. Kozak

Radoslaw P. Kozak studied chemistry at Warsaw University, Poland and obtained his Master of Science degree in 2007 working on synthesis of hydrindane precursors of vitamins D with modifi ed side chains. In 2008, he joined Ludger Ltd. where he works as a Scientist and he is involved in chemical glycan release methodology and glycan derivitization by fl uorophore and chromophore tagging for MS and HPLC; glycosylation analysis and glycoprofi ling. In 2010, he started his PhD thesis in collaboration with the Department of Parasitology at Leiden University Medical Centre working on development of analytical methods for O-glycosylation analysis of recombinantly expressed proteins under the supervision of Prof. Andre M. Deelder and Dr. Manfred Wuhrer.

Richard A. Gardner

Dr. Richard A. Gardner studied Biological Chemistry at the University of Leicester before gaining his PhD in Bioorganic Chemistry, also at Leicester. After a postdoctoral position in Organic Chemistry at the University of Central Florida, Orlando, he returned to the UK to work for Evotec OAI in the Development section. After a year developing alternative routes to synthesize final compounds and large scale synthesis of APIs, he moved to his current position as Senior Scientist at Ludger Ltd. The main focus of his activities at Ludger include; research and development into new glycan release and glycan labelling kits, high throughput analysis of glycoproteins and glycans and SPE clean up methodologies.

Daryl L. Fernandes

Dr. Daryl Fernandes is the founder and Chief Executive of Ludger. Daryl has over twenty-nine years experience in development of glycoanalysis methods for glycoproteins of the immune system and biopharmaceuticals. He gained his doctorate at the Glycobiology Institute, University of Oxford and went on to become Process Development Manager and then Head of Analytical Services at Oxford Glycosciences (OGS). Daryl left OGS to found Ludger in 1999. His interests at Ludger include the development and manufacture of reliable biopharmaceutical glycoprofi ling technology and improving the methods for design and production of glycosylated biopharmaceuticals.

André M. Deelder

Prof. Dr. André M. Deelder graduated in Biology in 1971 at the University of Leiden. He performed his PhD research at the department of Parasitology and received his PhD-degree in 1973. In 1985 he was appointed professor in Parasitology and head of the Department of Parasitology at the LUMC. He is leading the Biomolecular Mass Spectrometry Unit with research in clinical proteomics, metabolomics, glycomics and lipidomics. André has published extensively in the fi elds of molecular parasitology, proteomics and applied and translational research.

Manfred Wuhrer

Dr. Manfred Wuhrer studied biochemistry in Regensburg, Germany. He did his PhD (1999) and a subsequent postdoc in the group of Prof. Rudolf Geyer at Giessen University focusing on structural analysis of glycoconjugates. In 2003 he joined the group of Prof. Deelder at Leiden University Medical Center, The Netherlands. He works there currently as an associate professor and is heading the Glycomics and Glycoproteomics group. He is coordinator of the HighGlycan FP7 consortium dealing with the development of highthroughput glycoanalytical techniques for the characterization of biopharmaceuticals as well as for clinical and biological research.


Corresponding author


Received: 2012-06-05

Accepted: 2012-06-05

Published in Print: 2012-08-01


Citation Information: , Volume 393, Issue 8, Pages 687–708, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/hsz-2012-0144.

Export Citation

©2012 by Walter de Gruyter Berlin Boston.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Zsuzsanna Darula, Adam Pap, and Katalin F. Medzihradszky
Journal of Proteome Research, 2018
[2]
Djuro Josic, Tamara Martinovic, and Kresimir Pavelic
ELECTROPHORESIS, 2018
[3]
Shuang Yang, Philip Onigman, Wells W. Wu, Jonathan Sjogren, Helen Nyhlen, Rong-Fong Shen, and John Cipollo
Analytical Chemistry, 2018
[4]
Ieva Bagdonaite and Hans H Wandall
Glycobiology, 2018
[5]
Zsuzsanna Darula and Katalin F. Medzihradszky
Molecular & Cellular Proteomics, 2018, Volume 17, Number 1, Page 2
[6]
[7]
Unyong Kim, Myung Jin Oh, Youngsuk Seo, Yinae Jeon, Joon-Ho Eom, and Hyun Joo An
Bioanalysis, 2017
[8]
Vernon Reinhold, Hailong Zhang, Andrew Hanneman, and David Ashline
Molecular & Cellular Proteomics, 2013, Volume 12, Number 4, Page 866
[9]
Rebecca I. Thomson, Richard A. Gardner, Katja Strohfeldt, Daryl L. Fernandes, Graham P. Stafford, Daniel I. R. Spencer, and Helen M. I. Osborn
Analytical Chemistry, 2017, Volume 89, Number 12, Page 6455
[10]
Niclas G. Karlsson, Chunsheng Jin, Miguel A. Rojas-Macias, and Barbara Adamczyk
Trends in Glycoscience and Glycotechnology, 2017, Volume 29, Number 166, Page E35
[11]
SriHariRaju Mulagapati, Veerendra Koppolu, and T. Shantha Raju
Biochemistry, 2017, Volume 56, Number 9, Page 1218
[12]
Kenichiro Furuki, Toshimasa Toyo’oka, and Kazutoshi Ban
Analytical and Bioanalytical Chemistry, 2017, Volume 409, Number 9, Page 2269
[13]
Hongqiang Qin, Kai Cheng, Jun Zhu, Jiawei Mao, Fangjun Wang, Mingming Dong, Rui Chen, Zhimou Guo, Xinmiao Liang, Mingliang Ye, and Hanfa Zou
Analytical Chemistry, 2017, Volume 89, Number 3, Page 1469
[14]
Mengmeng Dai, Changming Yu, Ting Fang, Ling Fu, Jing Wang, Jun Zhang, Jun Ren, Junjie Xu, Xiaopeng Zhang, Wei Chen, and Salvatore V Pizzo
PLOS ONE, 2015, Volume 10, Number 12, Page e0145419
[15]
Radoslaw P. Kozak, Paulina A. Urbanowicz, Chamindie Punyadeera, Karli R. Reiding, Bas C. Jansen, Louise Royle, Daniel I. Spencer, Daryl L. Fernandes, Manfred Wuhrer, and Utpal Pal
PLOS ONE, 2016, Volume 11, Number 9, Page e0162824
[16]
Robbie P Joosten and Thomas Lütteke
Current Opinion in Structural Biology, 2017, Volume 44, Page 9
[17]
Ju-Wan Kim, Heeyoun Hwang, Jong-Sun Lim, Hyoung-Joo Lee, Seul-Ki Jeong, Jong Shin Yoo, and Young-Ki Paik
Journal of Proteome Research, 2016, Volume 15, Number 11, Page 4116
[18]
Sachio Yamamoto, Mitsuhiro Kinoshita, and Shigeo Suzuki
Journal of Pharmaceutical and Biomedical Analysis, 2016, Volume 130, Page 273
[19]
[21]
Viktoria Dotz, Rob Haselberg, Archana Shubhakar, Radoslaw P. Kozak, David Falck, Yoann Rombouts, Dietmar Reusch, Govert W. Somsen, Daryl L. Fernandes, and Manfred Wuhrer
TrAC Trends in Analytical Chemistry, 2015, Volume 73, Page 1
[22]
Zhikai Zhu and Heather Desaire
Annual Review of Analytical Chemistry, 2015, Volume 8, Number 1, Page 463
[23]
Katalin F. Medzihradszky, Krista Kaasik, and Robert J. Chalkley
Analytical Chemistry, 2015, Volume 87, Number 5, Page 3064
[24]
Radoslav Goldman and Miloslav Sanda
PROTEOMICS - Clinical Applications, 2015, Volume 9, Number 1-2, Page 17
[25]
Archana Shubhakar, Karli R. Reiding, Richard A. Gardner, Daniel I. R. Spencer, Daryl L. Fernandes, and Manfred Wuhrer
Chromatographia, 2015, Volume 78, Number 5-6, Page 321
[26]
Li-Juan Huang, Jen-Hui Lin, Jung-Heng Tsai, Yen-Yin Chu, Yen-Wen Chen, Shun-Li Chen, and Shu-Hui Chen
Journal of Chromatography A, 2014, Volume 1371, Page 136
[27]
Albert Barroso, Estela Giménez, Fernando Benavente, José Barbosa, and Victoria Sanz-Nebot
Analytica Chimica Acta, 2015, Volume 854, Page 169
[28]
Steven B. Levery, Catharina Steentoft, Adnan Halim, Yoshiki Narimatsu, Henrik Clausen, and Sergey Y. Vakhrushev
Biochimica et Biophysica Acta (BBA) - General Subjects, 2015, Volume 1850, Number 1, Page 33
[29]
Jan Sedzik, Jan Pawel Jastrzebski, and Marina Grandis
Journal of Neuroscience Research, 2015, Volume 93, Number 1, Page 1
[30]
R. Shyama Prasad Rao, Jay J. Thelen, and Ján A. Miernyk
Frontiers in Plant Science, 2014, Volume 5
[31]
[32]
Morten Thaysen-Andersen and Nicolle H. Packer
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2014, Volume 1844, Number 9, Page 1437
[33]
Jun-ichi Furukawa, Naoki Fujitani, and Yasuro Shinohara
Biomolecules, 2013, Volume 3, Number 1, Page 198
[34]
Mirela Sarbu, Roxana M. Ghiulai, and Alina D. Zamfir
Amino Acids, 2014, Volume 46, Number 7, Page 1625
[35]
Zsuzsanna Darula and Katalin F. Medzihradszky
Journal of The American Society for Mass Spectrometry, 2014, Volume 25, Number 6, Page 977
[36]
Radoslaw P. Kozak, Louise Royle, Richard A. Gardner, Albert Bondt, Daryl L. Fernandes, and Manfred Wuhrer
Analytical Biochemistry, 2014, Volume 453, Page 29
[37]
Stephane Houel, Mark Hilliard, Ying Qing Yu, Niaobh McLoughlin, Silvia Millan Martin, Pauline M. Rudd, Jonathan P. Williams, and Weibin Chen
Analytical Chemistry, 2014, Volume 86, Number 1, Page 576
[39]
Giuseppe Palmisano, Martin R. Larsen, Nicolle H. Packer, and Morten Thaysen-Andersen
RSC Advances, 2013, Volume 3, Number 45, Page 22706
[40]
Verena Richter, Marcel Kwiatkowski, Maryam Omidi, Azam Omidi, Wesley D Robertson, and Hartmut Schlüter
Pharmaceutical Bioprocessing, 2013, Volume 1, Number 4, Page 381
[41]
Lasse Fredriksen, Anders Moen, Alexei A Adzhubei, Geir Mathiesen, Vincent GH Eijsink, and Wolfgang Egge-Jacobsen
Glycobiology, 2013, Volume 23, Number 12, Page 1439
[42]
Zhikai Zhu, Xiaomeng Su, Daniel F. Clark, Eden P. Go, and Heather Desaire
Analytical Chemistry, 2013, Volume 85, Number 17, Page 8403
[43]
Peiqing Zhang, Tianhua Wang, Muriel Bardor, and Zhiwei Song
Pharmaceutical Bioprocessing, 2013, Volume 1, Number 1, Page 89
[44]
Miloslav Sanda, Petr Pompach, Julius Benicky, and Radoslav Goldman
ELECTROPHORESIS, 2013, Volume 34, Number 16, Page 2342
[45]
Catharina Steentoft, Sergey Y Vakhrushev, Hiren J Joshi, Yun Kong, Malene B Vester-Christensen, Katrine T-B G Schjoldager, Kirstine Lavrsen, Sally Dabelsteen, Nis B Pedersen, Lara Marcos-Silva, Ramneek Gupta, Eric Paul Bennett, Ulla Mandel, Søren Brunak, Hans H Wandall, Steven B Levery, and Henrik Clausen
The EMBO Journal, 2013, Volume 32, Number 10, Page 1478

Comments (0)

Please log in or register to comment.
Log in