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Licensed Unlicensed Requires Authentication Published by De Gruyter April 11, 2014

Low mRNA expression levels of kallikrein-related peptidase 4 (KLK4) predict short-term relapse in patients with laryngeal squamous cell carcinoma

  • Emmanouela Foteinou , Christos K. Kontos , Aris I. Giotakis and Andreas Scorilas EMAIL logo
From the journal Biological Chemistry

Abstract

Several members of the family of tissue kallikrein and kallikrein-related peptidases have been suggested as promising tumor biomarkers with important prognostic significance. However, only one (KLK11) has already been studied in laryngeal squamous cell carcinoma (LSCC) as a potential biomarker for LSCC diagnosis and/or prognosis. Our study investigated the prognostic value of kallikrein-related peptidase-4 (KLK4) mRNA expression as a molecular tissue biomarker in LSCC. For this purpose, KLK4 mRNA expression analysis was performed in 116 cancerous and 74 paired non-cancerous laryngeal tissue specimens obtained from patients that had undergone surgical treatment for primary LSCC. A remarkable downregulation of KLK4 mRNA expression was discovered in laryngeal tumors, compared to non-cancerous laryngeal tissue specimens. KLK4 mRNA expression was also shown to distinguish LSCC from non-cancerous laryngeal tissues. Furthermore, low KLK4 mRNA expression was shown to predict poor disease-free survival, independently of the histological grade and size of the malignant tumor as well as patient TNM stage. According to Kaplan-Meier survival analysis, low KLK4 mRNA expression predicts short-term relapse even among patients with well-differentiated tumors or those at an early TNM stage. Thus, KLK4 mRNA positivity could be regarded as a novel independent indicator of favorable prognosis for the disease-free survival of LSCC patients.


Corresponding author: Andreas Scorilas, Department of Biochemistry and Molecular Biology, University of Athens, GR-15701 Athens, Greece, e-mail:

Acknowledgments

This work was financially supported by the Commission of the European Community through the INsPiRE project (EU-FP7-REGPOT-2011–1, proposal 284460).

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Received: 2014-2-15
Accepted: 2014-4-8
Published Online: 2014-4-11
Published in Print: 2014-9-1

©2014 by De Gruyter

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