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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

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Volume 396, Issue 4


microRNA-210 is involved in the regulation of postmenopausal osteoporosis through promotion of VEGF expression and osteoblast differentiation

Xiao-Dong Liu
  • Corresponding author
  • Department of Orthopedics, YangPu Hospital, TongJi University School of Medicine, #450 Tengyue Road, Shanghai 200090, China
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Feng Cai
  • Department of Orthopedics, YangPu Hospital, TongJi University School of Medicine, #1239 Siping Road, Shanghai 200090, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Liang Liu
  • Department of Orthopedics, YangPu Hospital, TongJi University School of Medicine, #1239 Siping Road, Shanghai 200090, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Yan Zhang
  • Department of Orthopedics, YangPu Hospital, TongJi University School of Medicine, #1239 Siping Road, Shanghai 200090, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ An-Li Yang
  • Department of Orthopedics, YangPu Hospital, TongJi University School of Medicine, #1239 Siping Road, Shanghai 200090, China
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-01-15 | DOI: https://doi.org/10.1515/hsz-2014-0268


MicroRNAs (miRNAs) are small non-protein-codingRNAs that function as negative gene expression regulators. miRNA-210 (miR-210) has recently been recognized in the pathogenesis of osteonecrosis associated with angiogenesis. Herein we aimed to explore the clinical significance of miR-210 treatment for postmenopausal osteoporosis. The expression of miR-210 was detected in bone marrow mesenchymal stem cells (BMSCs) in vitro and miR-210 significantly promoted the expression of vascular edothelial growth factor (VEGF) in BMSCs in a time-dependent manner (p<0.05). And miR-210 suppressed PPARγ expression but increased the expression of ALP and osterix, demonstrating that miR-210 inhibited adipocyte differentiation and promoted osteoblast differentiation of BMSCs in vitro. The protein expression of hypoxia-inducible factor 1 alpha (HIF-1α) and VEGF in 17β-estradiol (E2) treated osteoblasts were significantly increased in a dose- and time-dependent manner (p<0.05). And E2 inducted the VEGF expression through the PI3K/AKT signaling pathway in osteoblasts. Taken together, these data implied that miR-210 played an important role in ameliorating the estrogen deficiency caused-postmenopausal osteoporosis through promotion the VEGF expression and osteoblast differentiation.

Keywords: BMSC differentiation; miR-210; postmenopausal osteoporosis; VEGF


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About the article

Corresponding author: Xiao-Dong Liu, Department of Orthopedics, YangPu Hospital, TongJi University School of Medicine, #450 Tengyue Road, Shanghai 200090, China, e-mail:

aThese authors contributed equally to this work.

Received: 2014-11-03

Accepted: 2014-12-05

Published Online: 2015-01-15

Published in Print: 2015-04-01

Citation Information: Biological Chemistry, Volume 396, Issue 4, Pages 339–347, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/hsz-2014-0268.

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