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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Sies, Helmut / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred

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Volume 399, Issue 9


Novel splice variants of the human kallikrein-related peptidases 11 (KLK11) and 12 (KLK12), unraveled by next-generation sequencing technology

Panagiotis G. Adamopoulos
  • Department of Biochemistry and Molecular Biology, National and Kapodistrian University of Athens, Panepistimiopolis, GR-15701 Athens, Greece
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Christos K. KontosORCID iD: http://orcid.org/0000-0002-9935-8461 / Andreas ScorilasORCID iD: http://orcid.org/0000-0003-2427-4949
Published Online: 2018-06-06 | DOI: https://doi.org/10.1515/hsz-2017-0294


Tissue kallikrein, kallikrein-related peptidases (KLKs), and plasma kallikrein form the largest group of serine proteases in the human genome, sharing many structural and functional characteristics. In this study, we describe the molecular cloning of four novel splice variants of the human KLK11 and KLK12 genes, discovered by combining 3′ rapid amplification of cDNA ends (3′ RACE), next-generation sequencing (NGS) technology, advanced bioinformatic analysis and Sanger sequencing. Expression analysis of these new transcripts in cell lines originating from 17 cancerous and two normal tissues revealed the expression pattern of each transcript. These novel KLK11 and KLK12 splice variants represent new potential cancer biomarkers.

This article offers supplementary material which is provided at the end of the article.

Keywords: alternative splicing; alternative transcripts; kallikreins; NGS; serine proteases; splice variants


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About the article

Received: 2017-11-27

Accepted: 2018-04-15

Published Online: 2018-06-06

Published in Print: 2018-09-25

Citation Information: Biological Chemistry, Volume 399, Issue 9, Pages 1065–1071, ISSN (Online) 1437-4315, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/hsz-2017-0294.

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