Die Isolierung und chemische Charakterisierung des Hirudins

F. Markwardt

doi:10.1515/bchm2.1957.308.1.147

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Die Isolierung und chemische Charakterisierung des Hirudins

F. Markwardt

Published Online: 2009-11-24 | DOI: https://doi.org/10.1515/bchm2.1957.308.1.147

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Citation Information: Hoppe-Seyler´s Zeitschrift für physiologische Chemie, Volume 308, Issue 1, Pages 147–156, ISSN (Online) 1437-4315, ISSN (Print) 0018-4888, DOI: https://doi.org/10.1515/bchm2.1957.308.1.147.

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[18]

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[24]

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[34]

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[35]

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[36]

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[42]

Der Hirudintoleranztest

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DOI: 10.1007/BF01484579

[43]

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[44]

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[45]

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DOI: 10.1016/0014-5793(91)80659-Q

[46]

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DOI: 10.1007/BF03042629

[48]

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Robbert B. Wallis

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DOI: 10.1016/0165-6147(88)90124-1

[49]

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Song Ren, John W. Fenton, John M. Maraganore, Aubie Angel, and Garry X. Shen

Journal of Cardiovascular Pharmacology, 1997, Volume 29, Number 3, Page 337

DOI: 10.1097/00005344-199703000-00006

[50]

Reduction of r-hirudin induced bleeding in pigs by the administration of von Willebrand factor

G. Dickneite, H.-J. Friesen, G. Kumpe, and M. Reers

Platelets, 1996, Volume 7, Number 5-6, Page 283

DOI: 10.3109/09537109609023590

[51]

HIRUDIN: Clinical Potential of a Thrombin Inhibitor

Paul H. Johnson, Ph.D

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DOI: 10.1146/annurev.med.45.1.165

[52]

Über neue Proteaseinhibitoren mit breiter Wirkungsspezifität bei dem Polychaeten Sabellastarte indica (Savingny), I. Mitteilung

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DOI: 10.1515/cclm.1975.13.10.429

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