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Biomedical Glasses

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Novel antibacterial bioactive glass nanocomposite functionalized with tetracycline hydrochloride

Josefina Rivadeneira
  • Corresponding author
  • Grupo Interdisciplinario en Materiales-Universidad Católica de Salta (IESIINGUCASAL), Instituto de Tecnologías y Ciencias de Ingeniería- Universidad Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (INTECIN UBA-CONICET), Campo Castañares s/n, Salta, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Gisela M. Luz
  • Corresponding author
  • 3B’s Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, P-4806-909 Taipas, Guimarães, Portugal; ICVS/3B’s Associate Laboratory, Braga/Guimarães, Portugal
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/ M. Carina Audisio
  • Corresponding author
  • Instituto de Investigaciones para la Industria Química - Consejo Nacional de Investigaciones Científicas y Técnicas (INIQUI - CONICET), Universidad Nacional de Salta (UNSa), Av. Bolivia 5150, Salta, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ João F. Mano
  • Corresponding author
  • 3B’s Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, P-4806-909 Taipas, Guimarães, Portugal; ICVS/3B’s Associate Laboratory, Braga/Guimarães, Portugal
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  • De Gruyter OnlineGoogle Scholar
/ Alejandro A. Gorustovich
  • Corresponding author
  • Grupo Interdisciplinario en Materiales-Universidad Católica de Salta (IESIINGUCASAL), Instituto de Tecnologías y Ciencias de Ingeniería- Universidad Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (INTECIN UBA-CONICET), Campo Castañares s/n, Salta, Argentina
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2015-10-21 | DOI: https://doi.org/10.1515/bglass-2015-0012

Abstract

To prevent the high frequency of wound infections, anti-bacterial agents can be loaded onto composites. In the present study, the antibiotic tetracycline hydrochloride (TC)was incorporated, for the first time, in collagen type I membranes coated with nano-sized SiO2-CaOP2O5 bioactive glass (n-BG) obtained by a sol-gel chemical route.

Collagen membranes coated with n-BG were immersed in simulated body fluid (SBF) containing 0.25, 0.75 or 1.25 mg mL−1 of TC for 48 h at 37∘C following a coprecipitation method. The antibiotic was released in distilledwater at 37∘C for up to 72 h. The antibacterial activity of the composites was evaluated in vitro by the inhibition zone test and plate count method. Two different Staphylococcus aureus strains, S. aureus ATCC29213 and S. aureus ATCC25923, were exposed to the biomaterials. The results showed that the incorporation but not the release of TC was dependent on the initial concentration of TC in SBF. The biomaterials inhibited S. aureus growth, although the efficacy was similar for all the concentrations. The results allow us to conclude that the new composite could have potential in the prevention of wound infections.

Keywords: Tetracycline hydrochloride; bioactive glass; collagen; Staphylococcus

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About the article

Received: 2015-08-27

Accepted: 2015-09-02

Published Online: 2015-10-21


Citation Information: Biomedical glasses, Volume 1, Issue 1, ISSN (Online) 2299-3932, DOI: https://doi.org/10.1515/bglass-2015-0012.

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© 2015 J. Rivadeneira et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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[2]
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