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BY-NC-ND 4.0 license Open Access Published by De Gruyter Open Access February 2, 2018

The effect of serum proteins on apatite growth for 45S5 Bioglass and common sol-gel derived glass in SBF

  • Sen Lin and Julian R. Jones EMAIL logo
From the journal Biomedical Glasses

Abstract

The inhibitive effects of serum proteins on apatite growth was compared between melt-derived 45S5 Bioglass® and sol-gel derived bioactive glass of the 70S30C (70 mol% SiO2, 30 mol% CaO). By using techniques of XRD, TEM and Raman spectroscopy, the transformation of amorphous calcium phosphate to crystalline apatite, and the resulting size and aspect ratio of the crystals, in simulated body fluid (SBF), was seen to decrease in the presence of serum. XRD showed more rapid HA formation on Bioglass particles, compared to that forming on 70S30C particles, however TEM showed similar size and frequency of the needle-like crystals. Phosphate reduction in SBF was similar for Bioglass and 70S30C. Calcium carbonate formation was more likely on the phosphate-free sol-gel glass than on Bioglass.

References

[1] Hench, L.L., Splinter, R.J., Allen, W.C. and Greenlee, T.K., Bonding mechanisms at the interface of ceramic prosthetic materials, J Biomed Mater Res Symp, 1971, 2, 117-14110.1002/jbm.820050611Search in Google Scholar

[2] Hench, L.L., Opening paper 2015- Some comments on Bioglass: Four Eras of Discovery and Development, Biomedical Glasses, 2015, 1, 1-1110.1515/bglass-2015-0001Search in Google Scholar

[3] Xynos, I.D., Hukkanen, M.V.J., Batten, J.J., Buttery, L.D., Hench, L.L. and Polak, J.M., Bioglass 45S5 stimulates osteoblast turnover and enhances bone formation in vitro: Implications and applications for bone tissue engineering, Calcified tissue international, 2000, 67, 321-32910.1007/s002230001134Search in Google Scholar

[4] Jones, J.R., Brauer, D.S., Hupa, L. and Greenspan, D.C., Bioglass and bioactive glasses and their impact on healthcare, Int J Appl Glass Sci, 2016, 7, 423-43410.1111/ijag.12252Search in Google Scholar

[5] Miguez-Pacheco, V., Hench, L.L. and Boccaccini, A.R., Bioactive glasses beyond bone and teeth: Emerging applications in contact with soft tissues, Acta Biomater, 2015, 13, 1-1510.1016/j.actbio.2014.11.004Search in Google Scholar

[6] Jones, J.R., Review of bioactive glass: From Hench to hybrids, Acta Biomater, 2013, 9, 4457-448610.1016/j.actbio.2012.08.023Search in Google Scholar

[7] Kokubo, T., et al., Ca, P-rich layer formed on high-strength bioactive glass-Ceramic A-W, J. Biomed. Mater. Res., 1990, 24, 331-34310.1002/jbm.820240306Search in Google Scholar

[8] Kokubo, T. and Takadama, H., How useful is SBF in predicting in vivo bone bioactivity?, Biomaterials, 2006, 27, 2907-291510.1016/j.biomaterials.2006.01.017Search in Google Scholar PubMed

[9] Macon, A.L.B., et al., A unified in vitro evaluation for apatiteforming ability of bioactive glasses and their variants, J. Mater. Sci. - Mater. Med., 2015, 26, 115-11510.1007/s10856-015-5403-9Search in Google Scholar

[10] Radin, S., Ducheyne, P., Rothman, B. and Conti, A., The effect of in vitro modeling conditions on the surface reactions of bioactive glass, J. Biomed. Mater. Res., 1997, 37, 363-37510.1002/(SICI)1097-4636(19971205)37:3<363::AID-JBM7>3.0.CO;2-JSearch in Google Scholar

[11] Li, R., Clark, A.E. and Hench, L.L., An investigation of bioactive glass powders by sol-gel processing, Journal of Applied Biomaterials, 1991, 2, 231-23910.1002/jab.770020403Search in Google Scholar

[12] Pereira, M.M., Clark, A.E. and Hench, L.L., Calcium-Phosphate Formation on Sol-Gel-Derived Bioactive Glasses in-Vitro, J. Biomed. Mater. Res., 1994, 28, 693-69810.1002/jbm.820280606Search in Google Scholar

[13] Saravanapavan, P., Jones, J.R., Pryce, R.S. and Hench, L.L., Bioactivity of gel-glass powders in the CaO-SiO2 system: A comparison with ternary (CaO-P2O5-SiO2) and quaternary glasses (SiO2-CaO-P2O5-Na2O), J. Biomed. Mater. Res. Part A, 2003, 66A, 110-11910.1002/jbm.a.10532Search in Google Scholar

[14] Jones, J.R., Ehrenfried, L.M. and Hench, L.L., Optimising bioactive glass scaffolds for bone tissue engineering, Biomaterials, 2006, 27, 964-97310.1016/j.biomaterials.2005.07.017Search in Google Scholar

[15] Poologasundarampillai, G., Lee, P.D., Lam, C., Kourkouta, A.M. and Jones, J.R., Compressive Strength of Bioactive Sol-Gel Glass Foam Scaffolds, Int J Appl Glass Sci, 2016, 7, 229-23710.1111/ijag.12211Search in Google Scholar

[16] Lin, S., Ionescu, C., Pike, K.J., Smith, M.E. and Jones, J.R., Nanostructure evolution and calcium distribution in sol-gel derived bioactive glass, Journal of Materials Chemistry, 2009, 19, 1276- 128210.1039/b814292kSearch in Google Scholar

[17] Kokubo, T., Kushitani, H., Sakka, S., Kitsugi, T. and Yamamuro, T., Solution able to reproduce in vivo surface-structure in bioactive glass-ceramic A-W3, J. Biomed. Mater. Res., 1990, 24, 721- 73410.1002/jbm.820240607Search in Google Scholar

[18] Hench, L.L., Bioceramics: From Concept to Clinic, Journal of the American Ceramic Society, 1991, 74, 1487-151010.1111/j.1151-2916.1991.tb07132.xSearch in Google Scholar

[19] Vallet-Regi, M., Romero, A.M., Ragel, C.V. and LeGeros, R.Z., XRD, SEM-EDS, and FTIR studies of in vitro growth of an apatitelike layer on sol-gel glasses, Journal of BiomedicalMaterials Research, 1999, 44, 416-42110.1002/(SICI)1097-4636(19990315)44:4<416::AID-JBM7>3.0.CO;2-SSearch in Google Scholar

[20] Hill, R.G. and Brauer, D.S., Predicting the bioactivity of glasses using the network connectivity or split network models, J. Non- Cryst. Solids, 2011, 357, 3884-388710.1016/j.jnoncrysol.2011.07.025Search in Google Scholar

[21] Nommeots-Nomm, A., et al., Highly degradable porous meltderived bioactive glass foam scaffolds for bone regeneration, Acta Biomater, 2017, 57, 449-46110.1016/j.actbio.2017.04.030Search in Google Scholar PubMed

[22] Lin, S., Van den Bergh, W., Baker, S. and Jones, J.R., Protein interactions with nanoporous sol-gel derived bioactive glasses, Acta Biomater, 2011, 7, 3606-361510.1016/j.actbio.2011.06.042Search in Google Scholar PubMed

[23] Sepulveda, P., Jones, J.R. and Hench, L.L., Characterization of melt-derived 45S5 and sol-gel-derived 58S bioactive glasses, J. Biomed. Mater. Res., 2001, 58, 734-74010.1002/jbm.10026Search in Google Scholar PubMed

[24] Sepulveda, P., Jones, J.R. and Hench, L.L., In vitro dissolution of melt-derived 45S5 and sol-gel derived 58S bioactive glasses, J. Biomed. Mater. Res., 2002, 61, 301-31110.1002/jbm.10207Search in Google Scholar PubMed

[25] Jones, J.R., Sepulveda, P. and Hench, L.L., Dose-dependent behavior of bioactive glass dissolution, J. Biomed. Mater. Res., 2001, 58, 720-72610.1002/jbm.10053Search in Google Scholar PubMed

[26] Midha, S., van den Bergh,W., Kim, T.B., Lee, P.D., Jones, J.R. and Mitchell, C.A., Bioactive Glass Foam Scaffolds are Remodelled by Osteoclasts and Support the Formation of MineralizedMatrix and Vascular Networks In Vitro, Adv. Healthc. Mater., 2013, 2, 490-49910.1002/adhm.201200140Search in Google Scholar PubMed

[27] Porter, A.E., Botelho, C.M., Lopes, M.A., Santos, J.D., Best, S.M. and Bonfield, W., Ultrastructural comparison of dissolution and apatite precipitation on hydroxyapatite and silicon-substituted hydroxyapatite <I>in vitro</I> and <I>in vivo</I>, Journal of Biomedical Materials Research Part A, 2004, 69A, 670-67910.1002/jbm.a.30035Search in Google Scholar PubMed

[28] Midha, S., Kim, T.B., van den Bergh,W., Lee, P.D., Jones, J.R. and Mitchell, C.A., Preconditioned 70S30C bioactive glass foams promote osteogenesis in vivo, Acta Biomater, 2013, 9, 9169- 918210.1016/j.actbio.2013.07.014Search in Google Scholar PubMed

[29] Sauer, G.R., Zunic, W.B., Durig, J.R. and Wuthier, R.E., Fourier transform raman spectroscopy of synthetic and biological calcium phosphates, Calcified Tissue International, 1994, 54, 414- 42010.1007/BF00305529Search in Google Scholar PubMed

[30] Notingher, I., et al., Application of FTIR and Raman spectroscopy to characterisation of bioactive materials and living cells, Spectroscopy-an International Journal, 2003, 17, 275-28810.1155/2003/893584Search in Google Scholar

[31] Yuan, P., He, H.P., Wu, D.Q., Wang, D.Q. and Chen, L.J., Characterization of diatomaceous silica by Raman spectroscopy, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2004, 60, 2941-294510.1016/j.saa.2004.02.005Search in Google Scholar PubMed

[32] FitzGerald, V., et al., In situ high-energy X-ray diffraction study of a bioactive calciumsilicate foam immersed in simulated body fluid, Journal of Synchrotron Radiation, 2007, 14, 492-49910.1107/S0909049507042173Search in Google Scholar PubMed

Received: 2017-11-08
Revised: 2017-12-05
Accepted: 2017-12-10
Published Online: 2018-02-02

© 2018

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

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