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Bioethanol

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Cross-species PCR and field studies on Paulownia elongata: A potential bioenergy crop

Chhandak Basu
  • Corresponding author
  • Department of Biology, California State University, Northridge, 18111 Nordhoff Street, Northridge, California 91330, USA
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/ Nirmal Joshee
  • Corresponding author
  • Graduate Program in Biotechnology, Fort Valley State University, Fort Valley, Georgia 31030, USA
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/ Tigran Gezalian
  • Corresponding author
  • UCLA Harbor Medical Center, 1000W. Carson Street, Torrance, California 90502, USA
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/ Brajesh Nanda Vaidya
  • Corresponding author
  • Graduate Program in Biotechnology, Fort Valley State University, Fort Valley, Georgia 31030, USA
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/ Asada Satidkit
  • Corresponding author
  • Baxter Bioscience, 1700Rancho Cojejo Blvd., Thousand Oaks, California 91320, USA
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/ Homa Hemmati
  • Corresponding author
  • Department of Biology, California State University, Northridge, 18111 Nordhoff Street, Northridge, California 91330, USA
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/ Zachary David Perry
  • Corresponding author
  • Graduate Program in Biotechnology, Fort Valley State University, Fort Valley, Georgia 31030, USA
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Published Online: 2015-12-18 | DOI: https://doi.org/10.1515/bioeth-2015-0002

Abstract

Paulownia elongata is a short-rotation fast growing tree and is known for high biomass accumulation and carbon sequestration potential. Optimization of protocols for nucleic acid extraction, PCR, RT-PCR, and other molecular biology techniques are required for better understanding of cellulose synthesis and to assess the potential of Paulownia as a biofuel tree. The main objective of this work was to study a putative cellulose synthase amplicon expression under various environmental conditions and evaluate the potentials of Paulownia as a biofuel tree. Using cross-species PCR an amplicon representative of a putative cellulose synthase gene from Paulownia was identified. This 177-bp long DNA sequence was 46% similar with cellulose synthase genes from Arabidopsis as expected. Gene specific primers for this particular Paulownia cellulose synthase gene were designed and reverse transcription PCR was performed to confirm its transcription. We report an inexpensive cDNA dot-blot method to study expression of this gene under various environmental conditions. We observed that cold and, to a lesser extent, heat stress downregulated its expression. This information will help to understand cellulose deposition in plant cell wall under stressful conditions. To the best of our knowledge this is the first characterization of a cDNA sequence from Paulownia elongata.

This article offers supplementary material which is provided at the end of the article.

Keywords: biomass; carbon sequestration; cDNA; cellulose synthase; cross-species PCR; Paulownia elongata

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About the article

Received: 2014-10-28

Accepted: 2015-05-04

Published Online: 2015-12-18

Published in Print: 2016-01-01


Citation Information: Bioethanol, Volume 2, Issue 1, ISSN (Online) 2299-6788, DOI: https://doi.org/10.1515/bioeth-2015-0002.

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© 2015 Chhandak Basu, et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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