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Bioethanol

Ed. by Ruiz, Héctor

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Emerging Science

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2299-6788
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Optimization of pre-treatment of de-oiled oil seed cake for release of reducing sugars by response surface methodology

N Sharmada
  • Corresponding author
  • Department of Biotechnology Engineering, NMAM Institute of Technology, Nitte 574 110, Udupi district, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Apoorva Punja
  • Corresponding author
  • Department of Biotechnology Engineering, NMAM Institute of Technology, Nitte 574 110, Udupi district, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sonali S Shetty
  • Corresponding author
  • Department of Biotechnology Engineering, NMAM Institute of Technology, Nitte 574 110, Udupi district, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Vinayaka B Shet
  • Corresponding author
  • Department of Biotechnology Engineering, NMAM Institute of Technology, Nitte 574 110, Udupi district, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Louella Concepta Goveas
  • Corresponding author
  • Department of Biotechnology Engineering, NMAM Institute of Technology, Nitte 574 110, Udupi district, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ C Vaman Rao
  • Corresponding author
  • Department of Biotechnology Engineering, NMAM Institute of Technology, Nitte 574 110, Udupi district, India
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-01-29 | DOI: https://doi.org/10.1515/bioeth-2016-0006

Abstract

Pre-treatment is a process that releases simple sugars from complex lignocellulosic biomass by using chemicals like acid and alkali which are one of the simplest and cost effective techniques. In this study, the conditions for sulphuric acid and sodium hydroxide pretreatment of de-oiled oil seed cake (DOSC) were optimized by response surface methodology (RSM). The levels of factors (DOSC concentration, agitation speed, sulphuric acid (H2SO4), sodium hydroxide (NaOH) concentration and reaction time) that affect release of reducing sugars by pre-treatment were obtained by one factor at a time (OFAT) approach of which only H2SO4 concentration, NaOH concentration and reaction time showed significance. The levels of factors were optimized by central composite design. Optimized conditions were found to be 11.65% (v/v) of H2SO4 concentration at 1.28h and, 4 N of NaOH at 3.7 h for acid and alkali hydrolysis respectively. Under optimized conditions, the release of reducing sugars was found to be 0.69 g/L (41.36 mg RRS/ g cellulose) and 0.40 g/L (23.98 mg RRS/ g cellulose) for acid hydrolysis and alkali hydrolysis of DOSC, respectively. Hence, RSM was found to be an efficient technique to optimize the hydrolysis process and ensure maximum release of reducing sugars.

Keywords: pre-treatment; sulphuric acid; sodium hydroxide; de-oiled oil seed cake; lignocellulosic biomass

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About the article

Received: 2014-11-12

Accepted: 2015-07-28

Published Online: 2016-01-29


Citation Information: Bioethanol, Volume 2, Issue 1, ISSN (Online) 2299-6788, DOI: https://doi.org/10.1515/bioeth-2016-0006.

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© 2016 Sharmada N et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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