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Optimization of the laccase detoxification step in hybrid hydrolysis and fermentation processes from wheat straw by K. marxianus CECT 10875

Alfredo Oliva-Taravilla
  • Corresponding author
  • IMDEA Energy Institute, Biotechnological Processes for Energy Production Unit, 28935 Móstoles, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Elia Tomás-Pejó
  • Corresponding author
  • IMDEA Energy Institute, Biotechnological Processes for Energy Production Unit, 28935 Móstoles, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Marie Demuez
  • Corresponding author
  • IMDEA Energy Institute, Biotechnological Processes for Energy Production Unit, 28935 Móstoles, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Cristina González-Fernández
  • Corresponding author
  • IMDEA Energy Institute, Biotechnological Processes for Energy Production Unit, 28935 Móstoles, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Mercedes Ballesteros
  • Corresponding author
  • IMDEA Energy Institute, Biotechnological Processes for Energy Production Unit, 28935 Móstoles, Spain
  • CIEMAT, Renewable Energy Division, Biofuels Unit, 28040 Madrid, Spain
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2016-06-29 | DOI: https://doi.org/10.1515/bioeth-2016-0009


The addition of laccase enzymes reduces the amount of phenols present in lignocellulosic pretreated materials and increases their fermentability. However, laccase addition in combination with cellulases reduces hydrolysis yields. In this work, hybrid hydrolysis and fermentation (HHF) configuration allowed overcoming the negative effect of laccase treatment on enzymatic hydrolysis. Furthermore, the effects of different laccase dosages, length of detoxification time and inoculum size on ethanol production were evaluated. In the evaluated configurations, the different laccase dosages did not show any significant effect on enzymatic hydrolysis. The lowest laccase dosage (0.5 IU/g DW) removed ~70% of total phenols which was enough to reach the highest ethanol production yields (~10 g/L) using K. marxianus CECT 10875. Shorter detoxification times and larger inoculum sizes had a positive impact on both ethanol production and volumetric productivity. These optimal detoxification conditions enable the fermentation of inhibitory slurries by reducing the overall time and cost of the process.

Keywords: Wheat straw; slurry; laccase detoxification; thermotolerant yeast; HHF process


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About the article

Received: 2016-02-09

Accepted: 2016-04-12

Published Online: 2016-06-29

Citation Information: Bioethanol, Volume 2, Issue 1, Pages 126–133, ISSN (Online) 2299-6788, DOI: https://doi.org/10.1515/bioeth-2016-0009.

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© 2016 Alfredo Oliva-Taravilla et al.. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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