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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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Volume 1, Issue 1


Volume 10 (2015)

A bioactive collagen-β tricalcium phosphate scaffold for tissue engineering

Elena Oprita / Lucia Moldovan / Oana Craciunescu / Wanda Buzgariu / Christu Tardei / Otilia Zarnescu
Published Online: 2006-03-01 | DOI: https://doi.org/10.2478/s11535-006-0005-7


Collagen-phosphate composites (COL/β-TCP) are novel materials that have the potential to be used as bone analogues. The aim of our study was to develop a porous bioactive material composed of type I collagen, the main bone protein and tricalcium phosphate, the mineral phase of natural bone, and investigate their in vitro biocompatibility in a human dermal fibroblast culture system. In order to obtain the bioactive materials, type I collagen was isolated from bovine tendon and characterized by physicochemical methods. β-TCP was obtained from calcium carbonate by thermal decomposition at 900 °C temperature. The powder was examined with X-ray diffraction. Two variants of COL/β-TCP scaffolds (P1 and P2) were prepared and examined by scanning electron microscopy. Our results revealed a microporous structure with small white aggregates of β-TCP, non-homogenous scattered in the collagen framework without any preferential orientation. The biocompatibility of the obtained scaffolds was tested by biochemical and histological methods on human fibroblast cultures. Both materials acted as good subtrates for human dermal fibroblast proliferation and migration.

Keywords: Collagen; tricalcium phosphate; sponges; composites; cell proliferation

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About the article

Published Online: 2006-03-01

Published in Print: 2006-03-01

Citation Information: Open Life Sciences, Volume 1, Issue 1, Pages 61–72, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-006-0005-7.

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© 2006 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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