Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

1 Issue per year


IMPACT FACTOR 2017: 0.764
5-year IMPACT FACTOR: 0.787

CiteScore 2017: 0.88

SCImago Journal Rank (SJR) 2017: 0.271
Source Normalized Impact per Paper (SNIP) 2017: 0.545

Open Access
Online
ISSN
2391-5412
See all formats and pricing
More options …
Volume 1, Issue 1

Issues

Volume 10 (2015)

A bioactive collagen-β tricalcium phosphate scaffold for tissue engineering

Elena Oprita / Lucia Moldovan / Oana Craciunescu / Wanda Buzgariu / Christu Tardei / Otilia Zarnescu
Published Online: 2006-03-01 | DOI: https://doi.org/10.2478/s11535-006-0005-7

Abstract

Collagen-phosphate composites (COL/β-TCP) are novel materials that have the potential to be used as bone analogues. The aim of our study was to develop a porous bioactive material composed of type I collagen, the main bone protein and tricalcium phosphate, the mineral phase of natural bone, and investigate their in vitro biocompatibility in a human dermal fibroblast culture system. In order to obtain the bioactive materials, type I collagen was isolated from bovine tendon and characterized by physicochemical methods. β-TCP was obtained from calcium carbonate by thermal decomposition at 900 °C temperature. The powder was examined with X-ray diffraction. Two variants of COL/β-TCP scaffolds (P1 and P2) were prepared and examined by scanning electron microscopy. Our results revealed a microporous structure with small white aggregates of β-TCP, non-homogenous scattered in the collagen framework without any preferential orientation. The biocompatibility of the obtained scaffolds was tested by biochemical and histological methods on human fibroblast cultures. Both materials acted as good subtrates for human dermal fibroblast proliferation and migration.

Keywords: Collagen; tricalcium phosphate; sponges; composites; cell proliferation

  • [1] M. Wirdmann-Al-Adham, R. Gutwald, G. Lauer, U. Huber and R. Schmelzeisen: “How to optimize seeding and culturing of human osteoblast-like cells on various biomaterials”, Biomaterials Vol. 23, (2002), pp. 3319–3328. http://dx.doi.org/10.1016/S0142-9612(02)00019-4CrossrefGoogle Scholar

  • [2] F.R.A.J. Rose and R.O.C. Oreffo: “Bone Tissue Engineering: Hope vs Hype”, Biochem. Biophys. Res. Commun., Vol. 292, (2002), pp. 1–7. http://dx.doi.org/10.1006/bbrc.2002.6519CrossrefGoogle Scholar

  • [3] D. Gotora and J.T. Czernuszka: “Mineralisation and cross-Linking Techniques to Improve the Mechanical Properties of Collagen-Calcium Phosphate (Coll-Cap) Composites to be used as Bone Analoques”, Eur. Cell Mater., Vol. 7(1), (2004), p. 55. Google Scholar

  • [4] W. Suchanek and M. Yoshimura: “Processing and properties of hydroxiapatite-based biomaterials for use as hard tissue replecement implants”, J. Mater. Res., Vol. 13(1), (1998), pp. 94–119. CrossrefGoogle Scholar

  • [5] A. Sihna, A. Ingle, K.R. Munim, S.N. Vaidya, B.P. Sharma and A.N. Bhisey: “Development of calcium phosphate based bioceramics”, Bull. Mater. Sci. Vol. 24(6), (2001), pp. 653–657. Google Scholar

  • [6] A.R. Vaccaro: “The Role of the Osteoconductive Scaffold in Synthetic Bone Graft”, Orthopedics, Vol. 25(5), (2002), pp. 571–578. Google Scholar

  • [7] V. Vicente, L. Meseguer, F. Martinez, A. Galian, J. Rodriguez, M. Alcaraz and M. Clavel: “Ultrastructural study of the osteointegration of bioceramics (whitlockite and composite beta-TCP-collagen) in rabbit bone”, Ultrastruct. Pathol., Vol. 20(2), (1996), pp. 179–188. Google Scholar

  • [8] G. Negroiu, L. Moldovan, M. Caloianu, N. Mirancea and D. Mirancea: “Collagen-chondroitin sulfate substrates conditioned as sponges and membranes”, Rev. Roum. Biochem. Vol. 29(1), (1992), pp. 23–28. Google Scholar

  • [9] L.A. Elson and W.T.J. Morgan: “Determination of total hexosamines”, Meth. Enzymol., Vol. 8, (1966), pp. 20–24. Google Scholar

  • [10] G. Chirita and M. Chirita: Collagen in animal skin, Editura Tehnica, Bucharest, 1993, p. 268 (in Romanian). Google Scholar

  • [11] L. Moldovan, E.I. Oprita, O. Craciunescu, C. Tardei, D. Bojin and O. Zarnescu: “Histochemical and scanning electron microscopic characterization of tricalcium phosphate-collagen conjugated sponges”, Roumanian Biotech. Lett., Vol. 9(5), (2004), pp. 1887–1893. Google Scholar

  • [12] K de Groot: “Bioceramics consisting of calcium phosphate salts”, Biomaterials, Vol. 1, (1980), pp. 47–50. http://dx.doi.org/10.1016/0142-9612(80)90059-9CrossrefGoogle Scholar

  • [13] T. Mosmann: “Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxic assays”, J. Immunol. Methods, Vol. 65, (1983), pp. 55–63. http://dx.doi.org/10.1016/0022-1759(83)90303-4CrossrefGoogle Scholar

  • [14] Y. Liu, D.A. Peterson, H. Kimura and D. Schubert: “Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction”, J. Neurochem., Vol. 69, (1997), pp. 581–593. http://dx.doi.org/10.1046/j.1471-4159.1997.69020581.xCrossrefGoogle Scholar

  • [15] A. John, L. Hong, Y. Ikada and Y. Tabata: “A trial to prepare biodegradable collagen-hydroxiapatite composite for bone repair”, J. Biomater. Sci. Polym. Ed., Vol. 12(6), (2001), pp. 689–705. http://dx.doi.org/10.1163/156856201316883485CrossrefGoogle Scholar

  • [16] S.S. Liao, F.Z. Cui, W. Zhang and Q.L. Feng: “Hierarchically biomimetic bone scaffolds materials:nano HA/collagen/PLA composite”, J. Biomed. Mater. Res. B. Appl. Biomater., Vol. 69(2), (2004), pp. 158–165. http://dx.doi.org/10.1002/jbm.b.20035CrossrefGoogle Scholar

  • [17] S.H. Parkish: “Bone Graft Substitutes: Past, Present, Future”, J. Postgrad. Med., Vol. 48(2), (2002), pp. 142–148. Google Scholar

  • [18] M. Ozawa, K. Tanaka, S. Morikawa, M. Chazono and K. Fujii: “Clinical study of the pure βttricalcium phosphate — Reports of 167 cases”, J. East. JPN Orthop. Traumatol., Vol. 12, (2000), pp. 409–413. Google Scholar

  • [19] E. Fernandez, F.J. Gil, M.P. Ginebra, F.C.M. Driessens, J.A. Planell and S.M. Best: “Calcium phosphate bone cements for clinical applications. Part I: solution chemistry”, J. Mater. Sci. Mater. Med., Vol. 10, (1999), pp. 169–176. http://dx.doi.org/10.1023/A:1008937507714CrossrefGoogle Scholar

  • [20] W. Renooji, A. Hoogendoorn, W.J. Visser, R.H.F. Lentferkink, M.G.J. Schimitz, H.V. Ieperen, S.J. Oldenburg, W.M. Janssen, L.M.A. Akkermans and P. Wittebol: “Bioresoption of ceramic strontium-85-labeled calcium phosphate implants in dog femora”, Clin. Orthop., Vol. 197, (1985), pp. 272–285. Google Scholar

  • [21] N. Kotobuki, K. Ioku, D. Kawagoe, H. Fujimori, S. Goto and H. Ohgushi: “Observation of osteogenic differentiation cascade of living mesenchimal stem cells on transparent hydroxyapatite ceramics”, Biomaterials, Vol. 26, (2005), pp. 779–785. http://dx.doi.org/10.1016/j.biomaterials.2004.03.020CrossrefGoogle Scholar

About the article

Published Online: 2006-03-01

Published in Print: 2006-03-01


Citation Information: Open Life Sciences, Volume 1, Issue 1, Pages 61–72, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-006-0005-7.

Export Citation

© 2006 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Burcu Sarikaya and Halil Murat Aydin
BioMed Research International, 2015, Volume 2015, Page 1
[2]
Bapi Sarker, Jasmin Hum, Showan N. Nazhat, and Aldo R. Boccaccini
Advanced Healthcare Materials, 2015, Volume 4, Number 2, Page 176
[3]
Eun-Jung Lee, Shin-Hee Jun, Hyoun-Ee Kim, and Young-Hag Koh
Journal of Biomedical Materials Research Part A, 2012, Volume 100A, Number 4, Page 841
[4]
Oana Craciunescu, Christu Tardei, Lucia Moldovan, and Otilia Zarnescu
Open Life Sciences, 2011, Volume 6, Number 3
[5]
Kuo-Yu Chen, Pei-Chi Shyu, Yueh-Sheng Chen, and Chun-Hsu Yao
Macromolecular Bioscience, 2008, Volume 8, Number 10, Page 942
[6]
Elena Oprita, Lucia Moldovan, Oana Craciunescu, and Otilia Zarnescu
Open Life Sciences, 2008, Volume 3, Number 1

Comments (0)

Please log in or register to comment.
Log in