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formerly Central European Journal of Biology

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The effect of aluminium on bioelectrical activity of the Nitellopsis obtusa cell membrane after H+-ATPase inhibition

Vilma Kisnierienë / Vidmantas Sakalauskas
Published Online: 2007-06-01 | DOI: https://doi.org/10.2478/s11535-007-0009-y

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Abstract

Aluminium induced membrane potential (Em) changes and potential changes during repolarization phase of the action potential (AP) in the internodal cells of Nitellopsis obtusa after blocking H+-ATPase activity by DCCD were investigated. Micromolar concentrations of DCCD are sufficient to give complete and irreversible inhibition of proton pumping. The membrane potential was measured by conventional glass-microelectrode technique. We found that the half-amplitude pulse duration differs significantly between standard conditions, after DCCD application, and after H+-ATPase blocking and subsequent Al3+ treatment: 4.9, 7.7 and 17.2 seconds, respectively. We propose that in the short term (2 hours) treatment of Al3+, the decrease in membrane potential was compensated for by H+-ATPase activity. Blocking H+-ATPase activity by DCCD can enhance the influence of Al3+ on the bioelectrical activity of cell membranes.

Keywords: Characeae; aluminum; H+-ATPase; bioelectrical activity; action potential

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About the article

Published Online: 2007-06-01

Published in Print: 2007-06-01

Citation Information: Open Life Sciences, Volume 2, Issue 2, Pages 222–232, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-007-0009-y.

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© 2007 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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