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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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Volume 3, Issue 1


Volume 10 (2015)

Degradation of naphthalene by thermophilic bacteria via a pathway, through protocatechuic acid

Audrius Bubinas
  • Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, LT-03101, Vilnius, Lithuania
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/ Gražina Giedraitytė
  • Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, LT-03101, Vilnius, Lithuania
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/ Lilija Kalėdienė
  • Department of Plant Physiology and Microbiology, Faculty of Natural Sciences, Vilnius University, LT-03101, Vilnius, Lithuania
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/ Ona Nivinskiene / Rita Butkiene
Published Online: 2008-03-01 | DOI: https://doi.org/10.2478/s11535-007-0042-x


A number of thermophilic bacteria capable of utilizing naphthalene as a sole source of carbon were isolated from a high-temperature oilfield in Lithuania. These isolates were able to utilize several other aromatic compounds, such as anthracene, benzene, phenol, benzene-1, 3-diol, protocatechuic acid as well. Thermophilic isolate G27 ascribed to Geobacillus genus was found to have a high aromatic compound degrading capacity. Spectrophotometric determination of enzyme activities in cell-free extracts revealed that the last aromatic ring fission enzyme in naphthalene biotransformation by Geobacillus sp. G27 was inducible via protocatechuate 3, 4-dioxygenase; no protocatechuate 4, 5-dioxygenase, protocatechuate 2, 3-dioxygenase activities were detected. Intermediates such as o-phthalic and protocatechuic acids detected in culture supernatant confirmed that the metabolism of naphthalene by Geobacillus sp. G27 can proceed through protocatechuic acid via ortho-cleavage pathway and thus differs from the pathways known for mesophilic bacteria.

Keywords: Thermophilic bacteria; Geobacillus; Naphthalene; Dioxygenases

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About the article

Published Online: 2008-03-01

Published in Print: 2008-03-01

Citation Information: Open Life Sciences, Volume 3, Issue 1, Pages 61–68, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-007-0042-x.

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© 2008 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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