Jump to ContentJump to Main Navigation
Show Summary Details

Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

1 Issue per year


IMPACT FACTOR increased in 2015: 0.814
5-year IMPACT FACTOR: 0.870

SCImago Journal Rank (SJR) 2015: 0.362
Source Normalized Impact per Paper (SNIP) 2015: 0.538
Impact per Publication (IPP) 2015: 0.929

Open Access
Online
ISSN
2391-5412
See all formats and pricing
Volume 3, Issue 2 (Jun 2008)

Issues

Chemical composition and antimicrobial activity of Thymus pannonicus All. (Lamiaceae) essential oil

Zoran Maksimović
  • Institute of Pharmacognosy, School of Pharmacy, University of Belgrade, 11221, Belgrade, Serbia
  • Email:
/ Marina Milenković
  • Institute of Microbiology and Immunology, School of Pharmacy, University of Belgrade, 11221, Belgrade, Serbia
  • Email:
/ Dragana Vučićević
  • Institute of Microbiology and Immunology, School of Pharmacy, University of Belgrade, 11221, Belgrade, Serbia
  • Email:
/ Mihailo Ristić
  • Institute for Medicinal Plant Research „Dr Josif Pančić“, 11000, Belgrade, Serbia
  • Email:
Published Online: 2008-04-03 | DOI: https://doi.org/10.2478/s11535-008-0013-x

Abstract

This paper presents the results of a study on chemical composition and antimicrobial activity of Thymus pannonicus All. (Lamiaceae) essential oil from Vojvodina province (north of Serbia). The investigated oil was hydrodistilled from a flowering plant and analysed by GC and GC-MS. Fifty-three constituents were identified (>97% of total oil), with geranial (41.42%, w/w) and neral (29.61%, w/w) as the most prominent. The antimicrobial activity of the oil was evaluated using agar disc diffusion and broth microdilution method against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli, two strains of Klebsiella pneumoniae and two strains of Candida albicans. The essential oil exhibited antimicrobial activity to varying degrees against all tested strains. The maximum activity of T. Pannonicus oil was observed against E. coli, S. aureus and both tested strains of C. Albicans (MIC = 50 µ/ml, each). Moderate activity was observed against P. aeruginosa and one of the tested strains of K. Pneumoniae (MIC = 200 µ/ml), while E. faecalis and the other strain of K. Pneumoniae expressed a higher degree of resistance (MIC > 200 µ/ml). This study confirms that essential oil of T. pannonicus possesses remarkable in vitro antimicrobial activity against several medicinally important pathogens. This is attributable to lemon-scented citral, a mixture of geranial and neral, which has well-documented antimicrobial activity against a range of bacteria and fungi.

Keywords: Thymus pannonicus; Essential oil composition; Antimicrobial activity; Geranial; Neral

  • [1] Dorman H.J., Deans S.G., Antimicrobial agents from plants: antibacterial activity of plant volatile oils, J. Appl. Microbiol., 2000, 88, 308–316 http://dx.doi.org/10.1046/j.1365-2672.2000.00969.x [Crossref]

  • [2] Cowan M., Plant products as antimicrobial agents, Clin. Microbiol. Rev., 1999, 12, 564–582

  • [3] Jalas J., Thymus L., in: Tutin T.G., Heywood V.H., Burges N.A., Moore D.M., Valentine D.H., Walters S.M., et al., Flora Europaea, Vol. 3, Cambridge University Press, Cambridge, 1972, 172–182

  • [4] Diklić N., Thymus L., In: Josifović M. (Ed.), Flora SR Srbije, Tome VI, Serbian Academy of Sciences and Arts, Belgrade, 1974, 475–509, (in Serbian)

  • [5] Morales R., Studies on the genus Thymus, Lamiales Newsletter, 1996, 4, 6–8

  • [6] Didry N., Dubreuil L., Pinkas M., Antibacterial activity of thymol, carvacrol and cinnamaldehyde alone or in combination, Pharmazie, 1993, 48, 301–304

  • [7] Didry N., Dubreuil L., Pinkas M., Activity of thymol, carvacrol, cinnamaldehyde and eugenol on oral bacteria, Pharm. Acta Helv., 1994, 69, 25–28 http://dx.doi.org/10.1016/0031-6865(94)90027-2 [Crossref]

  • [8] Horváth Gy., Kocsis B., Botz L., Németh J., Szabó L.Gy., Antibacterial activity of Thymus phenols by direct bioautography, Acta Biol. Szeged., 2002, 46, 145–146

  • [9] European Pharmacopoeia, 6th edition. Council of Europe — European Directorate for the Quality of Medicines, Strasbourg, 2007

  • [10] Panjković-Matanović V., Biljnogeografska analiza flore Vršačkih planina, Matica Srpska, Novi Sad, 1989, (in Serbian)

  • [11] Lakušić D., Blaženčić J., Ranđelović V., Butorac B., Vukojičić S., Zlatković B., et al., Staništa Srbije — priručnik sa opisima i osnovnim podacima, Ministry of Science and Environment Protection; Institute of Botany and Botanical Garden “Jevremovac”, University of Belgrade, Belgrade, 2005, (in Serbian, http://habitat.bio.bg.ac.yu or http://www.ekoserb.sr.gov.yu/projekti/stanista/)

  • [12] Zeremski M., Geomorfologija Vršačkih planina, Matica Srpska, Novi Sad, 1985, (in Serbian)

  • [13] Farmakopeja SFRJ, Pharmacopoea Jugoslavica IV, Federal Institute for Health Protection, Belgrade, 1984, (in Serbo-Croatian)

  • [14] Adams R.P., Identification of essential oil component by gas chromatography/quadrupole mass spectrometry, Allured Publishing Corporation, Carol Stream, USA, 2001

  • [15] Acar J.F., Goldstein F.W., Disc susceptibility test, In: Lorian V. (Ed.), Antibiotics in laboratory medicine, Williams & Wilkins, Baltimore, London, Los Angeles, Sydney, 1996, 1–52

  • [16] Candan F., Unlu M., Tepe B., Daferera D., Polissiou M., Sökmen A., et al., Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae), J. Ethnopharmacol., 2003, 87, 215–220 http://dx.doi.org/10.1016/S0378-8741(03)00149-1 [Crossref]

  • [17] Karuza-Stojaković Lj., Pavlović S., Živanović P., Todorović B., Količina i sastav etarskih ulja različitih vrsta roda Thymus L., Arh. farm., 1989, 39, 105–111, (in Serbian)

  • [18] Pluhár Zs., Héthelyi É., Kutta G., Kamondy L., Evaluation of environmental factors influencing essential oil quality of Thymus pannonicus All. and Thymus praecox Opiz, J. Herbs Spices Med. Plants, 2007, 3, 23–43 http://dx.doi.org/10.1300/J044v13n01_03 [Crossref]

  • [19] Kutta G., Pluhár Zs., Sárosi Sz., Yield and composition of supercritical fluid extracts of different Lamiaceae species, Int. J. Hort. Sci., 2007, 13, 79–82

  • [20] Tepe B., Sokmen M., Akpulat H.A., Daferera D., Polissiou M., Sokmen A., Antioxidative activity of the essential oils of Thymus sipyleus subsp. sipyleus var. sipyleus and Thymus sipyleus subsp. sipyleus var. rosulans, J. Food Eng., 2005, 66, 447–454 http://dx.doi.org/10.1016/j.jfoodeng.2004.04.015 [Crossref]

  • [21] Ložienė K., Venskutonis P.R., Influence of environmental and genetic factors on the stability of essential oil composition of Thymus pulegioides, Biochem. Syst. Ecol., 2005, 33, 517–525 http://dx.doi.org/10.1016/j.bse.2004.10.004 [Crossref]

  • [22] Stahl-Biskup E., Holthuijzen J., Essential oil and glycosidically bound volatiles of lemon-scented thyme, Thymus x citriodorus (Pers.) Schreb, Flavour Fragr. J., 1995, 10, 225–229 http://dx.doi.org/10.1002/ffj.2730100317 [Crossref]

  • [23] Lis-Balchin M., Deans S.G., Bioactivity of selected plant essential oils against Listeria monocytogenes, J. Appl. Microbiol., 1997, 82, 759–762 http://dx.doi.org/10.1046/j.1365-2672.1997.00153.x [Crossref]

  • [24] Díaz L.H., Jorge M.R., Actividad antimicrobiana de plantas que crecen en Cuba, Rev. Cub. Plant. Med., 2001, 2, 44–47, (in Spanish)

  • [25] Ohno T., Kita M., Yamaoka Y., Imamura S., Yamamoto T., Mitsufuji S., et al., Antimicrobial activity of essential oils against Helicobacter pylori, Helicobacter, 2003, 8, 207–215 http://dx.doi.org/10.1046/j.1523-5378.2003.00146.x [Crossref]

  • [26] Nguefack J., Budde B.B., Jakobsen M., Five essential oils from aromatic plants of Cameroon: their antibacterial activity and ability to permeabilize the cytoplasmic membrane of Listeria innocua examined by flow cytometry, Lett. Appl. Microbiol., 2004, 39, 395–400 http://dx.doi.org/10.1111/j.1472-765X.2004.01587.x [Crossref]

  • [27] Sartoratto A., Machado A.L.M., Delarmelina C., Figueira G.M., Duarte M.C.T., Rehder V.L.G., Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil, Braz. J. Microbiol., 2004, 35, 275–280 http://dx.doi.org/10.1590/S1517-83822004000300001 [Crossref]

About the article

Published Online: 2008-04-03

Published in Print: 2008-06-01


Citation Information: Open Life Sciences, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-008-0013-x. Export Citation

© 2008 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. (CC BY-NC-ND 3.0)

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Shahin Gavanji, Sayed R. Zaker, Zahra G. Nejad, Azizollah Bakhtari, Elham S. Bidabadi, and Behrouz Larki
Integrative Medicine Research, 2015
[2]
Sanja Ćavar Zeljković and Milka Maksimović
Phytochemistry Reviews, 2015, Volume 14, Number 3, Page 335
[3]
Filippo Maggi, Giovanni Caprioli, Fabrizio Papa, Gianni Sagratini, Sauro Vittori, Vladislav Kolarčik, and Pavol Mártonfi
Natural Product Research, 2014, Volume 28, Number 19, Page 1557
[4]
Jelena Arsenijević, Jelena Marković, Ivan Šoštarić, and Slavica Ražić
Plant Physiology and Biochemistry, 2013, Volume 71, Page 298
[5]
L. I. Alekseeva and I. V. Gruzdev
Russian Journal of Plant Physiology, 2012, Volume 59, Number 6, Page 818
[6]
Chetan M. Zade, Umesh D. Pete, Smita S. Patil, Jitendra D. Bhosale, Chakradhar D. Hadole, Meghraj S. Kadam, and Ratnamala S. Bendre
Journal of Environmental Science and Health, Part B, 2012, Volume 47, Number 2, Page 136
[7]
Alireza Rustaiee, Fatemeh Sefidkon, Seyed Mohammad Fakhr Tabatabaei, Reza Omidbaigi, and Seyed Fazel Mirahmadi
Journal of Essential Oil Research, 2011, Volume 23, Number 3, Page 6
[8]
Jelena Arsenijević, Slavica Ražić, Zoran Maksimović, and Svetlana Đogo
Open Life Sciences, 2011, Volume 6, Number 4
[9]
Urszula Kosikowska, Helena Smolarz, and Anna Malm
Open Life Sciences, 2010, Volume 5, Number 6

Comments (0)

Please log in or register to comment.
Log in