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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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IMPACT FACTOR 2016 (Open Life Sciences): 0.448

CiteScore 2016: 1.02

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2391-5412
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Volume 3, Issue 3

Issues

Effects of intermittent hypoxia different regimes on mitochondrial lipid peroxidation and glutathione-redox balance in stressed rats

Olga Gonchar
  • Department of Hypoxic States, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, 01024, Kyiv, Ukraine
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Published Online: 2008-07-12 | DOI: https://doi.org/10.2478/s11535-008-0016-7

Abstract

The purpose of this study was to compare the influence of two regimes of intermittent hypoxia (IH) [repetitive 5 cycles of 5 min hypoxia (7% O2 or 12% O2 in N2) followed by 15 min normoxia, daily for three weeks] on oxidative stress protective systems in liver mitochondria. To estimate the effectiveness of hypoxia adaptation at the early and late preconditioning period, we exposed rats to acute 6-h immobilization at the 1st and 45th days after cessation of IH. We showed that severity of hypoxic episodes during IH might initiate different adaptive programs. Moderate hypoxia during IH prevents mitochondrial glutathione pool depletion induced by immobilization stress, maintains GSH-redox cycle via activation of glutathione peroxidase, glutathione-S-transferase, glutathione reductase, NADP+-dependent isocitrate dehydrogenase, and increases Mn-SOD activity. Such regimen of hypoxic preconditioning caused the decrease of mitochondrial superoxide anion generation as well as of basal and stimulated in vitro lipid peroxidation and this protective effect remained for 45 days under renormoxic conditions. Hypoxic adaptation in a more severe regimen exerted beneficial effects on the mitochondrial antioxidant defense system only at its later phase.

Keywords: Intermittent hypoxia; Adaptation; Mitochondria; Lipid peroxidation; Glutathione; Glutathione enzymes; Antioxidant defense

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About the article

Published Online: 2008-07-12

Published in Print: 2008-09-01


Citation Information: Open Life Sciences, Volume 3, Issue 3, Pages 233–242, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-008-0016-7.

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© 2008 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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