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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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IMPACT FACTOR 2016 (Open Life Sciences): 0.448

CiteScore 2016: 1.02

SCImago Journal Rank (SJR) 2016: 0.329
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ISSN
2391-5412
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Volume 4, Issue 3 (Sep 2009)

Issues

The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells

Vilma Kisnierienė / Vidmantas Sakalauskas / Aleksandras Pleskačiauskas / Vladimir Yurin
  • Department of Plants Physiology and Biochemistry, Biological Faculty, Belarusian State University, 220050, Minsk, Belarus
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/ Osvaldas Rukšėnas
Published Online: 2009-07-26 | DOI: https://doi.org/10.2478/s11535-009-0028-y

Abstract

Interrelations between the action of acetylcholine (ACh) and cadmium ions (Cd2+) on bioelectrogenesis of Nitellopsis obtusa cells were investigated. We analyzed repetitively triggered action potentials (AP), their reproducibility, shape and dynamics of membrane potential after AP induction. ACh significantly increased membrane permeability only at high concentrations (1 mM and 5 mM). Repolarisation level of action potential after the first stimulus was much more positive in all cells treated with ACh as compared to the control. Differences of membrane potentials between points just before the first and the second stimuli were 23.4±.0 mV (control); 40.4±5.9 mV (1 mM ACh solution) and 57.7 ± 8.5 mV (5 mM ACh solution). Cd2+ at 20 μM concentration was examined as a possible inhibitor of acetylcholinesterase (AChE) in vivo. We found that cadmium strengthens depolarizing effect of acetylcholine after the first stimulus. The highest velocity of AP repolarization was reduced after ACh application and Cd2+strengthened this effect. There were no differences in dynamics of membrane potential after repetitively triggered action potentials in ACh or ACh and Cd2+ solutions. This shows that cadmium in small concentration acts as inhibitor of acetylcholinesterase.

Keywords: Action potential; Acetylcholine; Plant acetylcholinesterase; Plant electrical signaling; Characeae

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About the article

Published Online: 2009-07-26

Published in Print: 2009-09-01


Citation Information: Open Life Sciences, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-009-0028-y.

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© 2009 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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