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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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Volume 5, Issue 1

Issues

Volume 10 (2015)

De-intercalation of ethidium bromide and propidium iodine from DNA in the presence of caffeine

Jacek Piosik
  • Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, 80-822, Gdańsk, Poland
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/ Kacper Wasielewski
  • Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, 80-822, Gdańsk, Poland
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/ Anna Woziwodzka
  • Department of Molecular and Cellular Biology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, 80-822, Gdańsk, Poland
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/ Wojciech Śledź
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, 80-822, Gdańsk, Poland
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/ Anna Gwizdek-Wiśniewska
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology of University of Gdańsk and Medical University of Gdańsk, 80-822, Gdańsk, Poland
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Published Online: 2010-01-30 | DOI: https://doi.org/10.2478/s11535-009-0077-2

Abstract

Caffeine (CAF) is capable of interacting directly with several genotoxic aromatic ligands by stacking aggregation. Formation of such hetero-complexes may diminish pharmacological activity of these ligands, which is often related to its direct interaction with DNA. To check these interactions we performed three independent series of spectroscopic titrations for each ligand (ethidium bromide, EB, and propidium iodine, PI) according to the following setup: DNA with ligand, ligand with CAF and DNA-ligand mixture with CAF. We analyzed DNA-ligand and ligand-CAF mixtures numerically using well known models: McGhee-von Hippel model for ligand-DNA interactions and thermodynamic-statistical model of mixed association of caffeine with aromatic ligands developed by Zdunek et al. (2000). Based on these models we calculated association constants and concentrations of mixture components using a novel method developed here. Results are in good agreement with parameters calculated in separate experiments and demonstrate de-intercalation of EB and PI molecules from DNA caused by CAF.

Keywords: Caffeine; Ethidium bromide; Propidium iodine; De-intercalation; Interception; Light absorption spectroscopy; Genotoxic compounds

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About the article

Published Online: 2010-01-30

Published in Print: 2010-02-01


Citation Information: Open Life Sciences, Volume 5, Issue 1, Pages 59–66, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-009-0077-2.

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© 2010 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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