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Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz

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Volume 5, Issue 3


Volume 10 (2015)

Comparative effect of amidated pectin and psyllium on cholesterol homeostasis in rats

Milan Marounek
  • Institute of Animal Science, CZ-104 00, Prague, Czech Republic
  • Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, CZ-142 20, Prague, Czech Republic
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/ Zdeněk Volek / Eva Skřivanová / Jan Tůma / Dagmar Dušková
Published Online: 2010-04-08 | DOI: https://doi.org/10.2478/s11535-010-0014-4


The effects of amidated pectin and psyllium on serum, hepatic and faecal cholesterol concentration were compared in female rats fed diets supplemented with palm fat and cholesterol at 50 and 10 g/kg, respectively. Control rats were fed a diet supplemented with cellulose at 60 g/kg. In treated rats, cellulose was replaced with either amidated pectin or psyllium. Amidated pectin and psyllium intake significantly decreased serum cholesterol from 3.41 μmol/ml (control) to 1.68 and 2.04 μmol/ml, respectively, and hepatic cholesterol from 31.9 μmol/g (control) to 7.2 and 9.0 μmol/g, respectively. Histology with lipid-staining Sudan Black B revealed that liver tissue from control rats was infiltrated with lipids, but staining was absent in livers of treated rats. No hepatic pathophysiology was apparent in treated rats. Amidated pectin and psyllium intake significantly increased faecal fat content. Faecal cholesterol content was significantly increased in rats that were fed amidated pectin, and non-significantly increased in rats that were fed psyllium. Body weight and food intake did not differ among treatment groups. In conclusion, amidated pectin, a novel sequestrant of sterols, demonstrated a similar effect on rat serum and hepatic cholesterol concentration to psyllium, which is a well-established hypocholesterolaemic agent.

Keywords: Cholesterol; Amidated pectin; Psyllium; Rat

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About the article

Published Online: 2010-04-08

Published in Print: 2010-06-01

Citation Information: Open Life Sciences, Volume 5, Issue 3, Pages 299–303, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-010-0014-4.

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© 2010 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

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