Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Open Life Sciences

formerly Central European Journal of Biology

Editor-in-Chief: Ratajczak, Mariusz


IMPACT FACTOR 2017: 0.764
5-year IMPACT FACTOR: 0.787

CiteScore 2017: 0.88

SCImago Journal Rank (SJR) 2017: 0.271
Source Normalized Impact per Paper (SNIP) 2017: 0.545

ICV 2017: 154.48

Open Access
Online
ISSN
2391-5412
See all formats and pricing
More options …
Volume 5, Issue 4

Issues

Volume 10 (2015)

Effects of combining Beauveria bassiana and Nosema pyrausta on the mortality of Ostrinia nubilalis

Khaled Abdel Rahman / Marek Barta / Ľudovít Cagáň
  • Department of Plant Protection, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, 949 76, Nitra, Slovak Republic
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2010-05-28 | DOI: https://doi.org/10.2478/s11535-010-0035-z

Abstract

We tested the combined effect of the fungus Beauveria bassiana and the microsporidium Nosema pyrausta on the European corn borer larvae, Ostrinia nubilalis, in the laboratory. The first instar of O. nubilalis larvae was the most sensitive to the B. bassiana infection followed by the fifth, second, third, and fourth instar (LC50s were 4.91, 6.67, 7.13, 9.15, and 6.51 × 105 conidia/ml for the first to fifth instars, respectively). Mortality of each instar increases positively with concentration of conidia. When B. bassiana and N. pyrausta were used in combination, mortality increased significantly in all instars. Relative to the B. bassiana treatment alone, the B. bassiana + N. pyrausta treatment decreased the LC50s by 42.16%, 37.63%, 21.60%, 27.11%, and 33.95% for the first to fifth instars, respectively. The combined effects of the two pathogens were mostly additive. However, at the two highest concentrations the pathogens interacted synergistically in the first and second instar. Individuals that survived the B. bassiana and B. bassiana + N. pyrausta treatments and developed into adults had significantly shorter lifespans and females oviposited fewer eggs than non-exposed insects. The effects on the longevity and the egg production were most pronounced at high concentration of B. bassiana conidia.

Keywords: Beauveria bassiana; Nosema pyrausta; Ostrinia nubilalis; Mortality; Lifespan; Reproductive performance

  • [1] Mutuura A., Munroe E., Taxonomy and distribution of the European corn borer and allied species: genus Ostrinia (Lepidoptera: Pyralidae), Mem. Entomol. Soc. Can., 1970, 71, 1–112 Google Scholar

  • [2] Caffrey D.J., Worthley L.H., A progress report on the investigations of the European corn borer, US Dept. Agr. Bull. 1476, 1927 Google Scholar

  • [3] Cagáň Ľ., European corn borer, Ostrinia nubilalis Hbn. — the pest of maize in Slovakia, Assoc. prof. thesis, Slovak University of Agriculture, Nitra, Slovakia, 1993 Google Scholar

  • [4] Szoke C., Zsubori Z., Pok I., Racz F., Illes O., Szegedi I., Significance of the European corn borer (Ostrinia nubilalis Hubn.) in maize production, Acta Agron. Hung., 2002, 50, 447–461 http://dx.doi.org/10.1556/AAgr.50.2002.4.8CrossrefGoogle Scholar

  • [5] Dillehay B.L., Calvin D.D., Roth G.W., Hyde J.A., Kuldau G.A., Kratochvil R.J., et al., Verification of a European corn borer (Lepidoptera: Crambidae) loss equation in the major corn production region of the Northeastern United States, J. Econ. Entomol., 2005, 98, 103–112 http://dx.doi.org/10.1603/0022-0493-98.1.103CrossrefGoogle Scholar

  • [6] Siegel J.P., The epizootiology of Nosema pyrausta (Paillot) in the European corn borer Ostrinia nubilalis, PhD thesis, University of Illinois, Illinois, USA, 1985 Google Scholar

  • [7] Lewis L.C., Cossentine J.E., Season long intraplant epizootics of entomopathogens, Beauveria bassiana and Nosema pyrausta, in a corn agroecosystem, Entomophaga, 1986, 34, 363–369 http://dx.doi.org/10.1007/BF02373152CrossrefGoogle Scholar

  • [8] Sajap A.S., Lewis L.C., Chronology of infection of European corn borer, (Lepidoptera: Pyralidae) with the microsporidium Nosema pyrausta: Effect on development and vertical transmission, Environ. Entomol., 1992, 21, 178–182 CrossrefGoogle Scholar

  • [9] Phoofolo M.W., Obrycki J.J., Lewis L.C., Quantitative assessment of biotic mortality factors of the European corn borer (Lepidoptera: Crambidae) in field corn, J. Econ. Entomol., 2001, 94, 617–622 http://dx.doi.org/10.1603/0022-0493-94.3.617CrossrefGoogle Scholar

  • [10] Hajek A.E., St Leger R.J., Interactions between fungal pathogens and insect hosts, Annu. Rev. Entomol., 1994, 39, 293–322 http://dx.doi.org/10.1146/annurev.en.39.010194.001453CrossrefGoogle Scholar

  • [11] Feng Z., Carruthers R.I., Roberts D.W., Robson D.S., Age-specific dose-mortality effects of Beauveria bassiana (Deuteromycotina: Hyphomycetes) on the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae), J. Invertebr. Pathol., 1985, 46, 259–264 http://dx.doi.org/10.1016/0022-2011(85)90067-9CrossrefGoogle Scholar

  • [12] Feng Z., Carruthers R.I., Larkin T.S., Roberts D.W., A phenology model and field evaluation of Beauveria bassiana (Bals.) Vuillemin (Deuteromycotina: Hymphomycetes) mycosis of the European corn borer, Ostrinia nubilalis (Hbn.) (Lepidoptera: Pyralidae), Can. Entomol., 1988, 120, 133–144 http://dx.doi.org/10.4039/Ent120133-2CrossrefGoogle Scholar

  • [13] Legaspi J.C., Poprawski T.J., Legaspi B.C., Laboratory and field evaluation of Beauveria bassiana against sugarcane stalkborers (Lepidoptera: Pyralidae) in the Lower Rio Grande Valley of Texas, J. Econ. Entomol., 2000, 93, 54–59 http://dx.doi.org/10.1603/0022-0493-93.1.54CrossrefGoogle Scholar

  • [14] Leland J.E., McGuire M.R., Grace J.A., Jaronski S.T., Ulloa M., Park Y.H., et al., Strain selection of a fungal entomopathogen, Beauveria bassiana, for control of plant bugs (Lygus spp.) (Heteroptera: Miridae), Biol. Control., 2005, 35, 104–114 http://dx.doi.org/10.1016/j.biocontrol.2005.06.005CrossrefGoogle Scholar

  • [15] Shah P.A., Pell J.K., Entomopathogenic fungi as biological control agents, Appl. Microbiol. Biotech., 2003, 61, 413–423 CrossrefGoogle Scholar

  • [16] Lewis L.C., Bing L.A., Bacillus thuringiensis and Beauveria bassiana (Balsamo) Vuillemin for European corn borer control: Program for immediate and season-long suppression, Can. Entomol., 1991, 123, 387–393 http://dx.doi.org/10.4039/Ent123387-2CrossrefGoogle Scholar

  • [17] Cagáň Ľ., Uhlík V., Pathogenicity of Beauveria bassiana strains isolated from Ostrinia nubilalis Hbn. (Lepidoptera: Pyralidae) to original host larvae and to ladybirds (Coleoptera: Coccinelidae), Plant. Protect. Sci., 1999, 35, 108–112 Google Scholar

  • [18] Lewis L.C., Bruck D.J., Gunnarson R.D., On-farm evaluation of Beauveria bassiana for control of Ostrinia nubilalis in Iowa, USA, Biocontrol, 2002, 47, 167–176 http://dx.doi.org/10.1023/A:1014574526071CrossrefGoogle Scholar

  • [19] Lewis L.C., Berry E.C., Obrycki J.J., Bing L.A., Aptness of insecticides (Bacillus thuringiensis and carbofuran) with endophytic Beauveria bassiana, in suppressing larval populations of the European corn borer, Agr. Ecosyst. Environ., 1996, 57, 27–34 http://dx.doi.org/10.1016/0167-8809(95)01011-4CrossrefGoogle Scholar

  • [20] Lewis L.C., Lublinkhof J., Berry E.C., Gunnarson R.D., Response of Ostrinia nubilalis (Lep. Pyralidae) infected with Nosema pyrausta (Microsporida: Nosematidae) to insecticides, Entomophaga, 1982, 27, 211–218 http://dx.doi.org/10.1007/BF02375231CrossrefGoogle Scholar

  • [21] Lewis L.C., Sumerford D.V., Bing L.A., Gunnarson R.D., Dynamics of Nosema pyrausta in natural populations of the European corn borer, Ostrinia nubilalis: A six-year study, Biocontrol, 2006, 51, 627–642 http://dx.doi.org/10.1007/s10526-005-2937-7CrossrefGoogle Scholar

  • [22] Siegel J.P., Maddox J.V., Ruesink W.G., Lethal and sublethal effects of Nosema pyrausta on the European corn borer (Ostrinia nubilalis) in central Illinois, J. Invertebr. Pathol., 1986, 48, 167–173 http://dx.doi.org/10.1016/0022-2011(86)90119-9CrossrefGoogle Scholar

  • [23] Bauer L.S., Miller D.L., Maddox J.V., McManus M.L., Interactions between a Nosema sp. (Microspora: Nosematidae) and nuclear polyhedrosis virus infecting the gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae), J. Invertebr. Pathol., 1998, 74, 147–153 http://dx.doi.org/10.1006/jipa.1998.4773CrossrefGoogle Scholar

  • [24] Lewis L.C., Bruck D.J., Prasifka J.R., Raun E.S., Nosema pyrausta: Its biology, history, and potential role in a landscape of transgenic insecticidal crops, Biol. Control, 2009, 48, 223–231 http://dx.doi.org/10.1016/j.biocontrol.2008.10.009CrossrefGoogle Scholar

  • [25] Maddox J.V., Protozoan diseases, In: Fuxa J.R., Tanada Y., (Eds.), Epizootiology of insect diseases, John Wiley & Sons, New York, 1987 Google Scholar

  • [26] Onstad D.W., Maddox J.V., Modeling the effects of the Microsporidium, Nosema pyrausta, on the population dynamics of the insect, Ostrinia nubilalis, J. Invert. Pathol., 1989, 53, 410–421 http://dx.doi.org/10.1016/0022-2011(89)90107-9CrossrefGoogle Scholar

  • [27] Onstad D.W., Carruthers R.I., Epizootiological models of insect diseases, Ann. Rev. Entomol., 1990, 35, 399–419 http://dx.doi.org/10.1146/annurev.en.35.010190.002151CrossrefGoogle Scholar

  • [28] Windels M.B., Chiang H.C., Furgal A.B., Effects of Nosema pyrausta on pupa and adult stages of the European corn borer, Ostrinia nubilalis, J. Invertebr. Pathol., 1976, 27, 239–242 http://dx.doi.org/10.1016/0022-2011(76)90151-8CrossrefGoogle Scholar

  • [29] Bruck D.J., Lewis L.C., Gunnarson R.D., Interaction of Nosema pyrausta and temperature on Ostrinia nubilalis egg production and hatch, J. Invertebr. Pathol., 2001, 78, 210–214 http://dx.doi.org/10.1006/jipa.2001.5062CrossrefGoogle Scholar

  • [30] Nagy B., Rearing of the European corn borer (Ostrinia nubilalis Hbn.) on a simplified artificial diet, Acta Phytopathol. Acad. Sci. Hung., 1974, 5, 73–79 Google Scholar

  • [31] Finney D.J., Probit analysis, 3rd ed., Cambridge University Press, London, 1971 Google Scholar

  • [32] Abbott W.S., A method of computing the effectiveness of an insecticide, J. Econ. Entomol., 1925, 18, 256–257 Google Scholar

  • [33] Robertson J.L., Preisler H.K., Pesticide bioassays with arthropods, CRC, Boca Raton, 1982 Google Scholar

  • [34] Abdel Hakim A., Pathogenic studies on certain lepidopterous insects, PhD thesis, Cairo University, Cairo, Egypt, 1996 Google Scholar

  • [35] Riba G., Marcandier S., Richard G., Larget I., Sensibilitéde la pyrale du maïs (Ostrinia nubilalis) (Lep.: Pyralidae) aux hypomycétes entomopathogènes, Entomophaga, 1983, 28, 55–64, (in French) http://dx.doi.org/10.1007/BF02372097CrossrefGoogle Scholar

  • [36] UhlÍk V., Utilization of entomogenous fungus Beauveria bassiana (Bals.) Vuill. in biological control of the European corn borer, Ostrinia nubilalis Hbn., PhD thesis, Slovak University of Agriculture, Nitra, Slovakia, 1999 Google Scholar

  • [37] Jacques R.P., Morris O.N., Compatibility of pathogens with other methods of pest control and with different crops, In: Burges H.D., Hussey N.W., (Eds.), Microbial Control of Insect and Mites, Academic Press, New York, 1981 Google Scholar

  • [38] Pierce C.M.F., Solter L.F., Weinzierl R.A., Interactions between Nosema pyrausta (Microsporidia: Nosematidae) and Bacillus thuringiensis subsp. kurstaki in the European corn borer (Lepidoptera: Pyralidae), J. Econ. Entomol., 2001, 94, 1361–1368 http://dx.doi.org/10.1603/0022-0493-94.6.1361CrossrefGoogle Scholar

  • [39] Reardon B.J., Hellmich R.L., Sumerford D.V., Lewis L.C., Growth, development, and survival of Nosema pyrausta-infected European corn borers (Lepidoptera: Crambidae) reared on meridic diet and Cry1Ab, J. Econ. Entomol., 2004, 97, 1198–1201 http://dx.doi.org/10.1603/0022-0493-97.4.1198Google Scholar

  • [40] Tounou A.K., Kooyman C., Douro-Kpindou O.K., Poehling H.M., Interaction between Paranosema locustae and Metarhizium anisopliae var. acridum, two pathogens of the desert locust, Schistocerca gregaria under laboratory conditions, J. Invertebr. Pathol., 2008, 97, 203–210 http://dx.doi.org/10.1016/j.jip.2007.10.002CrossrefGoogle Scholar

  • [41] Hafez M., Zaki F.N., Moursy A., Sabbour M., Biological effects of the entomopathogenic fungus, Beauveria bassiana on the potato tuber moth Phthorimaea operculella (Zeller), Anz. Schadlingskd. Pfl., 1997, 70, 158–159 CrossrefGoogle Scholar

  • [42] N’Doye M., Influence d’une infection a Beauveria bassiana (Blas.) Vuillemin sur les survivants et la descendance de Chilo suppressalis Walker (Lep. Pyralidae), Entomophaga, 1976, 21, 371–376, (in French) http://dx.doi.org/10.1007/BF02371636CrossrefGoogle Scholar

  • [43] Fargues J., Delmas J.C., Auge J., Lebrun R.A., Fecundity and egg fertility in the adult Colorado beetle (Leptinotarsa decemlineata) surviving larval infection by the fungus Beauveria bassiana, Entomol. Exp. Appl., 1991, 61, 45–51 http://dx.doi.org/10.1007/BF00367167CrossrefGoogle Scholar

  • [44] Mulock B.S., Chandler L.D., Effect of Beauveria bassiana on the fecundity of western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae), Biol. Control, 2001, 22, 16–21 http://dx.doi.org/10.1006/bcon.2001.0952CrossrefGoogle Scholar

  • [45] Sikura A.I., Sikura L.V.R., Trebesava M., Influence of white muscardine fungus (Beauveria bassiana Balsamo Vuillemin) on the reproductive system of the Colorado potato beetle, Zashch. Rast. Kichinev, 1972, 2, 89–97 Google Scholar

About the article

Published Online: 2010-05-28

Published in Print: 2010-08-01


Citation Information: Open Life Sciences, Volume 5, Issue 4, Pages 472–480, ISSN (Online) 2391-5412, DOI: https://doi.org/10.2478/s11535-010-0035-z.

Export Citation

© 2010 Versita Warsaw. This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. BY-NC-ND 3.0

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Spiridon Mantzoukas, Christos Chondrogiannis, and George Grammatikopoulos
Entomologia Experimentalis et Applicata, 2015, Volume 154, Number 1, Page 78
[2]
Spiridon Mantzoukas, Panagiotis Milonas, Dimitris Kontodimas, and Kostas Angelopoulos
Annals of Microbiology, 2013, Volume 63, Number 3, Page 1083
[3]
A.K. Tounou, C. Kooyman, O.K. Douro-Kpindou, Y.M. Gumedzoe, and H.M. Poehlingn
Biocontrol Science and Technology, 2011, Volume 21, Number 5, Page 605

Comments (0)

Please log in or register to comment.
Log in